牡丹花发育相关基因PsPI的克隆与表达分析  

Cloning and Expression Analysis of PsPI Gene in Tree Peony (Paeonia suffruticosa)

作　　者：郑赟 王昊宁 刘靖文 唐文龙 李永华[1] 栗燕[1] 逯久幸[1] Zheng Yun;Wang Haoning;Liu Jingwen;Tang Wenlong;Li Yonghua;Li Yan;Lu Jiuxing(College of Forestry,Henan Agricultural University,Zhengzhou,450002;College of International Education,Henan Agricultural University,Zhengzhou,450002)

机构地区：[1]河南农业大学林学院,郑州450002 [2]河南农业大学国际教育学院,郑州450002

出　　处：《分子植物育种》2021年第7期2185-2192,共8页Molecular Plant Breeding

基　　金：河南省高等学校重点科研项目计划(19A220003);河南农业大学创新基金(KJCX2018A05);河南省科技攻关项目(192102110062)共同资助。

摘　　要：PI基因能够调控植物花器官形成。本研究以牡丹品种‘洛阳红’为研究材料,通过克隆从牡丹花瓣中得到PI同源基因,并利用qRT-PCR对其表达特性进行分析。结果表明,牡丹PI基因cDNA全长636 bp,包含MADS MEF domain,K-box domain,氨基酸序列C末端具有典型PI-motif I,命名为PsPI,GenBank登录号为MH169595。序列比对与系统进化分析表明,牡丹PsPI与芍药亲缘关系最近,相似性达90%以上。PsPI在牡丹营养器官中痕量表达,生殖器官组织中的表达量较高,花瓣中表达量最高,为萼片32倍;牡丹花发育6个时期中,雄蕊、雌蕊原基分化期PsPI表达量与其他4个时期存在显著差异,雌蕊原基分化期最高;4种不同花型牡丹的花器官中,PsPI在花瓣中表达量高于萼片、雌蕊与雄蕊,单瓣型、荷花型牡丹表达量显著高于皇冠型与蔷薇型。PsPI基因参与雌蕊、雄蕊原基分化,不同花型牡丹中该基因均在花瓣表现出较高的表达量,在雌蕊、雄蕊瓣化过程中可能具有关键作用,进而调控牡丹花器官的形态建成,该结果为牡丹花型分子育种提供了理论依据。PI gene regulates formation of flower forms. A novel PI gene which belongs to MADS-box gene family was cloned from Paeonia suffruticosa cultivar ’Luoyanghong’ by RT-PCR technique, and the relative expression level was analyzed by qRT-PCR. The result showed that it contained 636 bp full length cDNA with highly conserved MADS MEF domain, K-box domain, typical PI-motif I and named as Ps PI(GenBank No: MH169595). Homology analysis showed that Ps PI protein had a high identity with PI from Paeonia lactiflora(90%). Expression analysis showed that Ps PI was highly expressed in reproductive organs and tissues especially in petal(32 times of sepal),and it had a trace level in the vegetative organs. During the six flower development stages, Ps PI had a significant difference between pistil and stamen differentiation stage to other four differentiation stages, and the highest expression was in the carpel differentiation stage. Ps PI was highly expressed in petal than sepal, stamen and pistil in four tree peony cultivars, and the expression in Simple form and Lotus f orm were significantly higher than Crown form and Rose form. In conclusion, PsPI probably regulates stamen and pistil petalization in tree peony, and it has a highly expression in petal in different peony forms. Therefore, it may play a vital role in pistil and stamen petalization, and then regulates the morphogenesis of peony flower organs. The results provide a theoretical basis for molecular breeding of peony flower type.

关 键 词：牡丹 花发育 PsPI 克隆 表达分析 

分 类 号：S685.11[农业科学—观赏园艺]