芒SWN1基因的同源克隆及生物信息学分析  

Homologous Cloning and Bioinformatics Analysis of Gene SWN1 in Miscanthus sinensis

作　　者：何彦岑 李遥 林宇环 李蒙 陈智勇 He Yancen;Li Yao;Lin Yuhuan;Li Meng;Chen Zhiyong(Hunan Engineering Laboratory for Eeological Application of Miscanthus Resources,College of Bioscience and Hunan Engineering and Biotechnology,Hunan Agricultural University,410128)

机构地区：[1]湖南农业大学生物科学技术学院,芒属植物生态应用技术湖南省工程实验室,长沙410128

出　　处：《分子植物育种》2021年第7期2228-2235,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31471557;31871693);长沙市科技局国际合作重点项目(kq1901031)共同资助。

摘　　要：植物次生壁生物合成的转录调控网络是由一系列次生壁NAC开关转录因子介导的。在该网络中,顶层主开关SWNs(secondary wall NACs)转录因子与二级主开关MYBs转录因子共同激活下游转录因子和次生壁生物合成基因的表达,并且在进化上具有保守性。为探究芒次生壁合成调控网络,本研究利用水稻次生壁合成调控基因OsSWN1(SECONDARYWALL NAC DOMAINPROTEIN 1 from Oryza sativa)通过同源克隆法扩增得到了芒MsSWN1基因的cDNA序列。序列分析显示MsSWN1基因编码区长1215 bp,编码404个氨基酸。所编码的蛋白质相对分子量为43181.39 kD,预测其等电点(pI)为6.57,属于亲水蛋白,具有保守的NAC结构域。通过二级结构和三级结构预测分析表明,α-螺旋及不规则卷曲是其蛋白质结构中的主要结构组成元件。序列分析和系统进化树分析显示,芒MsSWN1基因编码的蛋白与甘蔗和高粱亲缘关系最为接近,并且该基因在其他禾本科植物中也具有很高的保守性,推测芒MsSWN1基因可能也具有调控次生壁生物合成的功能。本研究旨在为进一步挖掘和验证芒MsSWN1基因功能提供依据,为未来构建芒次生壁合成调控网络和更高效生产生物燃料提供有益信息。Transcriptional regulation of secondary wall biosynthesis in plants is mediated by a group of secondary wall NAC master switches. In this network, the top-level master switches, secondary wall NACs(SWNs), act together with the second-level MYB master switches to turn on the expression of downstream transcription factors(TFs) and secondary wall biosynthetic genes, which is evolutionarily conserved. To investigate transcriptional regulation of secondary wall biosynthesis in Miscanthus sinensis, the homologous genes of OsSWN1(SECONDARY WALL NAC DOMAINPROTEIN 1 from Oryza sativa) from Miscanthus sinensis was cloned by a homology-based cloning method in this study, named MsSWN1. Bioinformatics analysis showed that the coding region of MsSWN1 gene is 1 215 bp and encoded 404 amino acids. The relative molecular weight of the encoded protein is 43 181.39 D,and its predicted isoelectric point(pI) is 6.57, which is a hydrophilic protein and has a conservative NAC domain.The secondary and tertiary structure prediction analysis showed that α-helix and irregular curl were the main structural elements in the overall protein structure. DNA sequence analysis and phylogenetic tree analysis showed that the protein encoded by MsSWN1 gene is highly conserved in sugarcane, sorghum, and other Gramineae plants,speculating that MsSWN1 gene may also play a regulational function in secondary wall biosynthesis. These results laid the foundation for further digging and verifying the function of MsSWN1 gene, aiming to provide useful information for the future construction of secondary wall biosynthesis regulatory network of Miscanthus sinensis and the efficient biofuel production.

关 键 词：芒 SWN1 次生壁合成调控 生物信息学 

分 类 号：S578[农业科学—作物学]