河北省19F型和19A型侵袭性肺炎链球菌耐药性分析及多位点序列分型分析  

Antibiotic resistance and multi-locus sequence typing of Streptococcus pneumoniae invasive serotypes 19F and 19A isolates in Hebei Province

作　　者：贾肇一[1] 何宝花[1] 王乐雨 张洪斌 张永茂[1] 孙印旗[1] JIA Zhao-yi;HE Bao-hua;WANG Le-yu;ZHANG Hong-bin;ZHANG Yong-mao;SUN Yin-qi(Hebei Provincial Key Laboratory of Pathogens and Epidemiology of Infectious Diseases,Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang,Hebei 050021,China)

机构地区：[1]河北省疾病预防控制中心河北省传染病病原鉴定分析与流行病学重点实验室(筹),石家庄050021

出　　处：《实用预防医学》2021年第5期546-549,共4页Practical Preventive Medicine

基　　金：河北省科技计划项目课题(16277745D);河北省科技支撑项目课题(14277733D)。

摘　　要：目的了解河北省侵袭性肺炎链球菌(Streptococcus pneumoniae, Spn)19F型和19A型的耐药特征及多位点序列分型(multilocus sequence type, MLST)分布。方法 19F型和19A型采用多重聚合酶链式反应(multiplex polymerase chain reaction, mPCR)进行筛选分析;采用微量营养肉汤稀释法对其进行11种常见药物的药物敏感性试验,测定其最低抑菌浓度值(minimum inhibitory concentration, MIC)并进行药物敏感性的判断;采用MLST技术对菌株进行序列分型分析。结果 15株19F型侵袭性Spn对利奈唑胺、万古霉素、左氧氟沙星呈现出完全敏感。9株19A型侵袭性Spn对氯霉素、利奈唑胺、万古霉素、头孢噻肟、左氧氟沙星呈现出完全敏感。15株19F型侵袭性Spn共检出4种序列分型(sequence type, ST),3株属于新的ST型,优势STs为ST-271(9株,60.00%);9株19A型侵袭性Spn共检出2种ST型,优势STs为ST-320(8株,88.89%)。结论本地区19F型和19A型侵袭性Spn耐药现象较为严重,且存在多重耐药;19F型的基因型具有多变性和复杂性,而19A型的基因型则较为紧凑。Objective To investigate the antibiotic resistance characteristics of Streptococcus pneumoniae invasive serotypes 19 F and 19 A isolates and the distribution of multi-locus sequence type(MLST) in Hebei Province. Methods Multiplex polymerase chain reaction(mPCR) assay was used to determine the serotypes 19 F and 19 A. Microdilution broth method was employed to test the antibiotic resistance to 11 drugs. The minimum inhibitory concentration(MIC) was determined, and the antibiotic resistance was analyzed. MLST was performed to determine the sequence types(STs) of serotypes 19 F and 19 A. Results Fifteen strains of Streptococcus pneumoniae invasive serotype 19 F were completely sensitive to linezolid, vancomycin and levofloxacin hydrochloride. Nine strains of Streptococcus pneumoniae invasive serotype 19 A were completely susceptible to chloromycetin, linezolid, vancomycin, cefotaxime and levofloxacin hydrochloride. Four STs were detected in fifteen strains of Streptococcus pneumoniae invasive serotype 19 F, three of which belonged to the new STs, and the dominant ST was ST-271(9 strains, 60.00%). Two STs were detected in nine strains of Streptococcus pneumoniae invasive serotype 19 A, and the dominant ST was ST-320(8 strains, 88.89%). Conclusion The antibiotic resistance of Streptococcus pneumoniae invasive serotypes 19 F and 19 A isolates in Hebei Province is serious, and there exists a multiple drug resistance pattern. The genotypes of Streptococcus pneumoniae invasive serotype 19 F are variable and complex, while the genotypes of Streptococcus pneumoniae invasive serotype 19 A are compact.

关 键 词：侵袭性肺炎链球菌 血清型19F 血清型19A 耐药性 多位点序列分型 

分 类 号：R563.1[医药卫生—呼吸系统]