Evaluating the Health Risks of Pneumonia from Airborne Bacterial Communities Using 16S rDNA Sequences of Pneumonia-related Pathogens  

作　　者：GUO Jian Guo KONG Qi LIU Ce KANG Tai Sheng QIN Chuan 

机构地区：[1]NHC Key Laboratory of Human Disease Comparative Medicine,Institute of Laboratory Animal Sciences,CAMS&PUMC,Beijing 100021,China [2]Key Laboratory of Human Diseases Animal Model,State Administration of Traditional Chinese Medicine,Beijing 100021,China [3]Department of Infectious Disease,Beijing Chuiyangliu Hospital affiliated to Tsinghua University,Beijing 100022,China

出　　处：《Biomedical and Environmental Sciences》2021年第4期265-271,共7页生物医学与环境科学（英文版）

基　　金：supported by the CAMS Innovation Fund for Medical Science[CIFMS,2018-I2M-1-001];the National Key R&D Program of China[2017YFC0702800];the National Natural Science Foundation of China[82070103];the Central Public-interest Scientific Institution Basal Research Fund[2016ZX310037]。

摘　　要：Objective Airborne microbial communities include a significant number of uncultured and poorly characterized bacteria.No effective method currently exists to evaluate the health risks of such complex bacterial populations,particularly for pneumonia.Methods We developed a method to evaluate risks from airborne microorganisms,guided by the principle that closer evolutionary relationships reflect similar biological characteristics,and thus used16 S rDNA sequences of 10 common pneumonia-related bacterial pathogens.We calculated a risk of breath-related(Rbr)index of airborne bacterial communities and verified effectiveness with artificial flora and a clinical project.Results We suggested applying Rbr80 to evaluate the health risks of airborne bacterial communities that comprise 80% of dominant operational taxonomic units(OTUs).The feasibility of Rbr80 was confirmed by artificial flora and by pneumonia data from a hospital.A high Rbr80 value indicated a high risk of pneumonia from airborne bacterial communities.Conclusion Rbr80 is an effective index to evaluate the pneumonia-associated risk from airborne bacteria.Values of Rbr80 greater than 15.40 are considered high risk.

关 键 词：Airborne bacteria The risk of breath related index(Rbr) PNEUMONIA Health risk 

分 类 号：R563.1[医药卫生—呼吸系统]