Assessment of Benchmark Dose in BEAS-2B Cells by Evaluating the Cell Relative Viability with Particulates in Motorcycle Exhaust via the Air-liquid Interface Exposure  

作　　者：YU Tao ZHANG Xue Yan LI Shu Fei ZHOU Yu Mei LI Bin WANG Zhong Xu DAI Yu Fei ADAMSON Sherleen Xue-Fu ZHENG Yu Xin BIN Ping 

机构地区：[1]National Institute of Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention,Beijing 100050,China [2]Tianjin Center for Diseases Prevention and Control,Tianjin 300011,China [3]School of Health Sciences,Purdue University,West Lafayette 47907,Indiana USA [4]School of Public Health,Qingdao University,Qingdao 266021,Shandong,China

出　　处：《Biomedical and Environmental Sciences》2021年第4期272-281,共10页生物医学与环境科学（英文版）

基　　金：supported by the funding from the National Natural Science Foundation of China[No.81472955,82073596 and 91643203]。

摘　　要：Objective This study aimed to use an air-liquid interface(ALI)exposure system to simulate the inhalation exposure of motorcycle exhaust particulates(MEPs)and then investigate the benchmark dose(BMD)of MEPs by evaluating cell relative viability(CRV)in lung epithelial BEAS-2B cells.Methods The MEPs dose was characterized by measuring the number concentration(NC),surface area concentration(SAC),and mass concentration(MC).BEAS-2B cells were exposed to MEPs at different concentrations via ALI and CRV was determined using Cell Counting Kit(CCK-8)assay.BMD software was applied to calculate BMD and the lower limit of benchmark dose(BMDL)according to Akaike Information Coefficient(AIC),with P-value based on Hill,Linear,Polynomial,and Power model.Results Our results reveal that BMD of NC and SAC were estimated by the best-fitting Hill model,while MC was estimated by Polynomial model.The BMDL for CRV following ALI exposure to MEPs were as follows:364.2#/cm^(3)for NC;0.662×10^(7)nm^(2)/cm^(3)for SAC;and 0.278μg/m^(3)for MC.Conclusion These results indicate that MEPs exposure via ALI system induces a dose-dependent decrease of CRV and provides the potential exposure threshold of MEPs in a lung cell model.

关 键 词：Motorcycle exhaust particulates Air-liquid interface Bronchial epithelial cells Cell relative viability Dose-dependent effect 

分 类 号：R114[医药卫生—卫生毒理学]