重组蛋白牛防御素5微球对BCG免疫小鼠的影响  

Effect of Nanoparticle-BNBD5 Complexes on BCG Immunized Mice

作　　者：刘一朵 于国际 张硕 梁正敏 孙星雅 赵德明[1] 周向梅[1] LIU Yi-duo;YU Guo-ji;ZHANG Shuo;LIANG Zheng-min;SUN Xing-ya;ZHAO De-ming;ZHOU Xiang-mei(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;Beijing Center for Animal Disease Control and Prevention,Beijing 102629,China)

机构地区：[1]中国农业大学动物医学院,北京海淀100193 [2]北京市动物疫病预防控制中心,北京大兴102629

出　　处：《中国兽医杂志》2020年第12期18-23,I0003,共7页Chinese Journal of Veterinary Medicine

基　　金：十三五重点研发项目(2017YFD0500901)。

摘　　要：为了研究牛防御素5(BNBD5)微球对卡介苗(BCG)免疫小鼠的影响,在表达纯化获得目的蛋白BNBD5后,制备包封BNBD5重组蛋白的聚乳酸-羟基乙酸(PLGA)纳米微球,并进行动物试验。用BCG对BALB/c小鼠免疫后,再用BNBD5纳米微粒对小鼠进行滴鼻,通过ELISA方法检测小鼠血清中细胞因子和抗体的变化,发现BNBD5-PLGA微球能够显著提高促炎因子IL-β(P<0.001)和TNF-α(P<0.001)以及抗体IgA(P<0.001)产生;再用牛分枝杆菌(Mycobacterium bovis)滴鼻感染小鼠,发现BNBD5-PLGA组临床症状和病理变化有所减轻。表明重组蛋白牛防御素5微球能刺激小鼠产生较高的非特异性细胞因子以及黏膜免疫抗体IgA水平,对黏膜免疫具有促进作用。To study the effect of bovine neutrophilsβ-defensins 5(BNBD5)nanoparticles on bacilli-calmette-guerin(BCG)immunized mice,we prepared the BNBD5-PLGA nanoparticle complexes and conducted animal experiments.After immunizing BALB/c mice with BCG,the mice were dripped with BNBD5 nanoparticles.From the results of cytokines and antibodies in the serum or lung lavage of mice by ELISA,we found that the BNBD5-PLGA microspheres significantly improved the production of pro-inflammatory factors IL-β(P<0.001),TNF-α(P<0.001)and IgA(P<0.001).The immunized mice were then challenged with Mycobacterium bovis.Clinical symptoms and pathological changes were relieved in the BNBD5-PLGA group.These data confirm that recombinant protein bovine defense 5 microspheres can stimulate mice to produce higher nonspecific cytokines and mucosal IgA levels,suggesting that it has a catalytic effect on mucosa immunity.

关 键 词：牛分枝杆菌 牛防御素5 聚乳酸-羟基乙酸 卡介苗 细胞因子 黏膜免疫 

分 类 号：S852.4[农业科学—基础兽医学]