5种布鲁氏菌病竞争ELISA抗体检测试剂盒的比较  

Comparison of 5 cELISA Kits for Detecting Antibodies of Brucellosis

作　　者：虞一聪 桂平雄 倪柏锋[1] 王雅婷 董建斐 周蕾[1] 柴娟[1] 谢荣辉[1] 赵灵燕[1] 徐辉[1] YU Yi-cong;GUI Ping-xiong;NI Bai-feng;WANG Ya-ting;DONG Jian-fei;ZHOU Lei;CHAI Juan;XIE Rong-hui;ZHAO Ling-yan;XU Hui(Zhejiang Provincial Center for Animal Disease Control,Hangzhou 311199,China)

机构地区：[1]浙江省动物疫病预防控制中心,浙江杭州311199

出　　处：《中国兽医杂志》2020年第12期36-39,共4页Chinese Journal of Veterinary Medicine

基　　金：浙江省农业重大技术协同推广计划(2019XTTGXM02-4);浙江省“三农六方”科技协作项目(CTZB-F180706LWZ-SNY1)。

摘　　要：为提高布鲁氏菌病检测诊断能力,采用5种布鲁氏菌病抗体竞争酶联免疫吸附试验(cELISA)试剂盒对背景清楚的166份牛、羊、猪血清样本进行检测,同时以试管凝集试验(SAT)作为参照方法,测定5种试剂盒的敏感性、特异性、Youden指数及AUC值等参数。结果显示,5种试剂盒的敏感性为88.00%~100.00%;特异性为86.21%~99.14%;Youden指数为86.21%~95.69%;AUC值为0.931~0.978;McNemar检验结果显示,5种试剂盒中厂家A、C和E检验结果与SAT结果差异不显著(P>0.05),厂家B和D检验结果与SAT结果差异显著(P<0.05);Kappa一致性检验分析表明,5种试剂盒与SAT结果均有高度的一致性(0.790~0.930);成本-效果分析表明,5种试剂盒优先选择依次为A、C、B、E、D。本试验结果为诊断用试剂盒选择提供参考,从而提高诊断准确性。To improve the ability of detection and diagnosis of brucellosis,five cELISA kits were used to detect 166 serum samples for brucellosis antibodies from cattle,sheep and pigs with a clear background.Meanwhile,the sensitivity,specificity,Youden’s J values and AUC values of the five kits were determined by SAT as a reference method.The results showed that the sensitivity of the five kits was 88.00%~100.00%;the specificity was 86.21%~99.14%;the Youden’s J value was 86.21%~95.69%;the AUC value was 0.931~0.978.McNemar test results showed no significant statistical difference was observed between the results of manufacturers A,C,and E with SAT results(P>0.05),while significant difference was observed between the SAT results of manufacturer B and D(P<0.05).Kappa consistency test analysis showed that the five kits were highly consistent with SAT results(0.790~0.930).The cost-effectiveness analysis showed that the priority of the five kits was A,C,B,E and D.These results will provide a reference for the selection of diagnostic kits,thereby improving the diagnostic accuracy.

关 键 词：布鲁氏菌病 竞争ELISA 试剂盒 比较 

分 类 号：R378.5[医药卫生—病原生物学]