妊娠期糖尿病患者血清和胎盘氧化应激相关蛋白表达水平与妊娠结局的关系  

Relationship between the expression of oxidative stress-related proteins in serum and placenta of pregnant women with gestational diabetes mellitus and pregnancy outcomes

作　　者：谈炯新[1] 李蕊 郭绮棱[1] 刘怀昌[1] 肖磊[1] TAN Jiongxin;LI Rui;GUO Qiling;LIU Huaichang;XIAO Lei(Department of Internal Medicine,Foshan Maternity and Child Health Hospital,Foshan,Guangdong,China,528000)

机构地区：[1]佛山市妇幼保健院内科,广东佛山528000

出　　处：《分子诊断与治疗杂志》2021年第4期631-634,共4页Journal of Molecular Diagnostics and Therapy

基　　金：广东省医学科学技术研究基金项目(A2019214);佛山市卫生和计生局医学科研(20180131)。

摘　　要：目的探讨妊娠期糖尿病患者血清和胎盘氧化应激相关蛋白表达水平与妊娠结局的关系。方法选取2017年8月至2019年6月于本院产科门诊接受孕期检查诊断为GDM的孕妇共108例(GDM组),选择90例正常孕妇作为对照组,记录两组的妊娠结局,采用ELISA测定所有孕妇的血清GSH、MDA和SOD1水平,以及采用免疫组化检测胎盘组织的SOD1、HO-1、NQO1和Nrf2表达水平,分析胎盘组织氧化应激相关蛋白表达水平与妊娠结局的关系。结果 GDM组孕妇的GSH和MDA指标显著低于对照组(P<0.05),且GDM组孕妇的SOD1指标显著高于对照组(P<0.05)。GDM组孕妇的胎盘SOD1与血清GSH、SOD呈显著的正相关(P<0.05),与血清MDA呈显著的负相关(P<0.05)。而胎盘HO-1、NQO1和Nrf2的表达水平均与血清GSH、SOD1呈显著的负相关,而与血清MDA呈显著的正相关(P<0.05),剖宫产孕妇的胎盘组织中SOD1表达水平显著低于非剖宫产孕妇(P<0.05),而HO-1、NQO1和Nrf2表达水平显著高于非剖宫产孕妇(P<0.05)。结论妊娠期糖尿病患者的血清和胎盘组织存在显著的氧化应激状态,胎盘组织的氧化应激指标与剖宫产及早产的发生密切相关。Objective To investigate the relationship between serum and placental oxidative stressrelated protein expression and pregnancy outcome in patients with gestational diabetes mellitus.Methods A total of 108 pregnant women who were diagnosed with GDM during pregnancy during the period from August2017 to June 2019 in our hospital were selected.90 normal pregnant women were selected as the control group.The pregnancy outcomes of 2 groups were recorded.Serum GSH,MDA and SOD1 levels were measured by ELISA in all pregnant women,and the expression levels of SOD1,HO-1,NQO1 and Nrf2 in placenta were detected by immunohistochemistry.The relationship between oxidative stress-related protein levels in placenta and pregnancy outcomes were analyzed.Results The GSH and MDA indicators of the GDM group were significantly lower than those of the control group(P<0.05),and the SOD1 level of the GDM group was significantly higher than that of the control group(P<0.05).There was a significant positive correlation between placental SOD1 and serum GSH and SOD1(P<0.05),and a significant negative correlation with serum MDA(P<0.05)in pregnant women with GDM.The expression levels of HO-1,NQO1 and Nrf2 in placenta were significantly negatively correlated with serum GSH and SOD1,but positively correlated with serum MDA(P<0.05).The expression level of SOD1 in placental tissues of cesarean section pregnant women was significantly lower than that in non-cesarean section pregnant women(P<0.05),and HO-1,NQO1 and Nrf2 expression levels were significantly higher than non-cesarean section pregnant women(P<0.05).Conclusion The serum and placental tissues of patients with gestational diabetes have significant oxidative stress.The oxidative stress indicators of placental tissue are closely related to the occurrence of cesarean section and premature delivery.

关 键 词：氧化应激 妊娠期糖尿病 妊娠结局 SOD1 HO-1 

分 类 号：R714.256[医药卫生—妇产科学]