利用流式细胞术对29份火龙果种质染色体的倍性鉴定  

Ploidy Determination of 29 Pitaya Germplasms Using Flow Cytometry

作　　者：黄黎芳[1] 武志江 梁桂东[1] 陆贵锋[1] 黄凤珠[1] 刘朝安 邓海燕[1] HUANG Lifang;WU Zhijiang;LIANG Guidong;LU Guifeng;HUANG Fengzhu;LIU Chaoan;DENG Haiyan(Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西壮族自治区农业科学院园艺研究所,广西南宁530007

出　　处：《热带作物学报》2021年第4期966-970,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31960578);广西特色水果创新团队项目(No.nycytxgxcxtd-17-13);广西创新驱动发展专项资金项目(桂科AA17204097-10)。

摘　　要：火龙果为仙人掌科量天尺属和蛇鞭柱属植物,不同类型火龙果种质的染色体倍性尚不明确。为明确所收集的种质资源以及创制的新材料的染色体倍性,利用流式细胞技术对29份火龙果材料进行了倍性鉴定。结果显示,供试的18份种质资源中共鉴定出二倍体火龙果14份,四倍体4份,无三倍体材料。通过不同倍性亲本杂交和秋水仙素诱变所创制的8份后代材料经鉴定发现有二倍体材料1份,三倍体材料3份,四倍体材料4份,这表明火龙果属间杂交和化学诱变可获得多倍体材料。研究结果对火龙果资源的遗传学评价以及开展倍性杂交育种提供重要的参考依据。Pitaya which belongs to the cactus family includes two genus,Hylocereus and Seleniereus,the ploidy levels of different pitaya germplasms are still not clear.In order to clarify the ploidy of germplasm resources and the hybrids or mutants,the ploidy of 29 pitaya materials were identified by flow cytometry.14 of 18 germplasm materials were diploid and others were tetraploid while no triploids were available.The 8 interploid hybrids and colchicine-induced mutants were identified as 3 triploids,4 tetraploids and 1 diploid,which showed that polyploid materials could be obtained by intergeneric hybridization and chemical mutagenesis of pitaya.The results would provide an important reference basis for genetic evaluation and ploidy cross breeding of pitaya resources.

关 键 词：流式细胞术 火龙果 种质资源 染色体 倍性分析 

分 类 号：S667[农业科学—果树学]