PCR技术在新冠病毒核酸检测中的应用  

Application of PCR in novel coronavirus nucleic acid detection

作　　者：张礼堃 邹秉杰(综述) 宋沁馨[2] 周国华(综述) ZHANG Li-kun;ZOU Bing-jie;SONG Qin-xin;ZHOU Guo-hua(Department of Clinical Pharmacy,Jinling Hospital,Nanjing University School of Medicine/General Hospital of Eastern Theater Command,PLA,Nanjing 210002,Jiangsu,China;School of Pharmacy,China Pharmaceutical University,Nanjing 210009,Jiangsu,China)

机构地区：[1]南京大学医学院附属金陵医院(东部战区总医院)临床药学科,南京210002 [2]中国药科大学药学院,南京210009

出　　处：《医学研究生学报》2021年第5期539-544,共6页Journal of Medical Postgraduates

基　　金：江苏省杰出青年基金项目(BK20180005);江苏省基础研究计划自然科学基金项目(BK20191322)。

摘　　要：自2019年12月底以来,由新型冠状病毒(SARS-CoV-2)感染引起的新冠肺炎(COVID-19)疫情在全世界范围内暴发。新冠病毒具有较强的传染性,因此快速、准确地检测新冠病毒是阻断疾病传播、防控新冠疫情的关键。目前国内外已经出现了多种新冠病毒核酸检测方法,其中聚合酶链式反应(PCR)是主流的方法。文章总结了新冠病毒核酸检测中以PCR为基础的方法,包括实时定量PCR、微滴式数字PCR、核酸即时检测技术、多重PCR,分析了这些方法的优缺点,并探讨了临床应用的实例。Since the end of December 2019,outbreaks of Covid-19 caused by novel coronavirus infection have been occurring worldwide.Novel coronavirus has a strong infectivity,so the rapid and accurate detection of novel coronavirus is the key to block the transmission of disease and prevent and control the new crown epidemic.Many novel coronavirus nucleic acid detection methods have appeared at home and abroad,among which the polymerase chain reaction(PCR)is the mainstream method.This paper summarized the PCR-based methods of novel coronavirus nucleic acid detection,including real-time quantitative PCR,microdroplet digital PCR,real-time nucleic acid detection technology and multiplex PCR,analyzed the advantages and disadvantages of these methods,and discussed the clinical application examples.

关 键 词：PCR技术 新型冠状病毒 核酸检测 

分 类 号：R511[医药卫生—内科学]