间隔区寡核苷酸DNA微阵列分型在结核分枝杆菌检测中的应用  

Application of spacer oligonucleotide typing base on DNA microarray genotyping in Mycobacterium tuberculosis

作　　者：杨建伟 李辉[2] 王少华[2] 杨卫华 杨俊梅 YANG Jianwei;LI Hui;WANG Shaohua;YANG Weihua;YANG Junmei(Henan Children′s Hospital,Zhengzhou Children′s Hospital,Zhengzhou Key Laboratory of Children′s Infection and Immunity,Zhengzhou 450018,Henan Province,China;Henan Center for Disease Control and Prevention,Zhengzhou 450016,Henan Province,China;Jiangsu Liezhen Biotechnology Limited Company,Rudong 226499,Jiangsu Province,China)

机构地区：[1]河南省儿童医院,郑州儿童医院,郑州市儿童感染与免疫重点实验室,河南郑州450018 [2]河南省疾病预防控制中心,河南郑州450016 [3]江苏猎阵生物科技有限公司,江苏如东226499

出　　处：《新乡医学院学报》2021年第4期323-327,共5页Journal of Xinxiang Medical University

基　　金：河南省科技发展计划项目(编号:162102310224);河南省医学科技攻关计划联合共建项目(编号:LHGJ20200666)。

摘　　要：目的探讨间隔区寡核苷酸DNA微阵列法在结核分枝杆菌基因分型检测中的应用价值。方法将结核分枝杆菌标准菌株(H37Rv)、卡介苗(BCG)菌株和97株临床结核分枝杆菌株的聚合酶链反应产物分别进行传统Spoligotyping和间隔区寡核苷酸DNA微阵列分型,比较2种方法的一致性,并用H37Rv菌株检测间隔区寡核苷酸DNA微阵列法的灵敏度。结果结核分枝杆菌标准菌株、BCG菌株和97株临床结核分枝杆菌菌株的间隔区寡核苷酸DNA微阵列分型结果与传统Spoligotyping完全一致,符合率为100%;且间隔区寡核苷酸DNA微阵列法有较好的检测效能。结论间隔区寡核苷酸DNA微阵列法用于结核分枝杆菌分型具有操作简便、快速等特点,适于结核病的溯源和分子流行病学调查。Objective To investigate the application value of spacer oligonucleotide typing(Spoligotyping)base on DNA microarray genotyping in Mycobacterium tuberculosis.Methods The polymerase chain reaction products of the standard strains of Mycobacterium tuberculosis,bacille calmette-guerin(BCG)and 97 strains of clinical Mycobacterium tuberculosis were detected with traditional Spoligotyping and DNA microarray method,respectively.The consistency of the two methods was compared,and the sensitivity of DNA microarray genotyping was detected by H37Rv strain.Results The results of spacer oligonucleotide DNA microarray typing of Mycobacterium tuberculosis standard strains,BCG strains and 97 strains of clinical Mycobacterium tuberculosis were completely consistent with that of traditional Spoligotyping,the coincidence rate was 100%,and the detection efficiency of Spoligotyping base on DNA microarray genotyping was better.Conclusion The Spoligotyping base on DNA microarray in the typing of Mycobacterium tuberculosis is simple and rapid,which is suitable for the traceability and molecular epidemiological investigation of tuberculosis.

关 键 词：结核分枝杆菌 DNA微阵列 间隔区寡核苷酸分型 

分 类 号：R52[医药卫生—内科学]