胆木浸膏糖浆质量标准研究  

Study on quality standard of Danmu extract syrup

作　　者：李湘怡 周明艳 谢振蕊 李丽[1] 刘爱霞 张钰昕 赖伟勇[1,2] 张俊清[1,2] 关薇薇[1] LI Xiang-yi;ZHOU Ming-yan;XIE Zhen-rui;LI Li;LIU Ai-xia;ZHANG Yu-xin;LAI Wei-yong;ZHANG Jun-qin;GUAN Wei-wei(Hainan Provincial Key Laboratory of R&D on Tropical Herbs,Haikou 571199,China;School of Pharmaceutical Sciences Hainan Medicine University,Haikou 571199,China)

机构地区：[1]海南医学院海南省热带药用植物研究开发重点实验室,海南海口571199 [2]海南医学院药学院,海南海口571199

出　　处：《海南医学院学报》2021年第9期651-655,660,共6页Journal of Hainan Medical University

基　　金：海南省重大科技计划项目(ZDKJ201805)。

摘　　要：目的:建立中药制剂胆木浸膏糖浆的质量标准。方法:采用薄层色谱法对胆木浸膏糖浆中胆木进行定性鉴别;采用高效液相色谱法测定胆木中的异长春花苷内酰胺和绿原酸的含量。结果:胆木薄层色谱鉴别斑点清晰、无阴性干扰。异长春花苷内酰胺在100~300μg/mL范围内线性关系良好(R2=0.9997),平均回收率为99.1%,RSD为1.16%(n=9);绿原酸在6.7~20.2μg/mL范围内线性关系良好(R2=0.9991),平均回收率为99.9%,RSD为1.10%(n=9)。结论:制定的胆木浸膏糖浆质量标准安全合理,方法简便,且准确性、重现性较好,可用于胆木浸膏糖浆的质量控制。Objective:To establish the quality standard of Danmu extract syrup.Methods:TLC method was used to quali-tatively identify Nauclea officinalis in Danmu extract syrup.The content of strictosamide and chlorogenic acid in Danmu extract syrup was determined by high efficiency axillary chromatography.Results:The spots were clear and there was no negative interference.The linear relationship of strictosamide was good in the range of 100-300μg/mL(R2=0.9997).The average recovery was 99.1%and RSD was 1.16%(n=9).Chlorogenic acid had a good linear relationship in the range of 6.7-20.2μg/mL(R2=0.9991).The average recovery was 99.9%and RSD was 1.10%(n=9).Conclusion:The standard is safe and reasonable,the method is simple,accurate and reproducible,and it can be used for the quality control of Danmu extract syrup.

关 键 词：胆木浸膏糖浆 质量标准 异长春花内酰胺 绿原酸 高效液相色谱法 薄层色谱法 

分 类 号：R286.0[医药卫生—中药学]