基于VEGFR2介导的Ras/MAPK信号通路探讨补肾安胎冲剂治疗复发性流产的作用机制  

VEGFR2 mediated Ras/MAPK signaling pathway to explore the mechanism of Bushen Antai Granule in the treatment of recurrent abortion

作　　者：常韦 李伟莉[2] 陆莎莎 CHANG Wei;LI Wei-li;LU Sha-sha(Anhui University of Traditional Chinese Medicine,Hefei,Anhui 230012,China;Department of Obstetrics and Gynecology,The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei,Anhui 230031,China)

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]安徽中医药大学第一附属医院妇产科,安徽合肥230031

出　　处：《海南医学院学报》2021年第9期672-678,共7页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(81574017)。

摘　　要：目的:探讨补肾安胎冲剂对小干扰RNA(small interfering RNA,siRNA)干扰的血管内皮生长因子受体2介导的Ras蛋白(rat sarcoma protein,Ras)/丝裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)mRNA及蛋白表达的影响。方法:构建胎盘微血管内皮细胞,并制备补肾安胎冲剂含药血清,筛选出最佳含药血清浓度,将其分为正常血清组、siRNA-NC正常血清组、药物血清组、siRNA正常血清组、siRNA药物血清组,采用实时荧光定量PCR、Western blotting、免疫荧光法分别检测Ras/MAPK mRNA及蛋白表达。结果:正常组和阴性对照组Ras、MAPK mRNA及蛋白的表达无明显差异(P>0.05);药物血清组细胞Ras、MAPK mRNA及蛋白的表达明显高于正常血清组(P<0.01);siRNA1正常血清组较正常血清组相比Ras、MAPK mRNA及蛋白的表达明显下降(P<0.01);siRNA1药物血清组与siRNA1正常血清组Ras、MAPK mRNA及蛋白的表达相比有显著差异(P<0.01)。结论:补肾安胎冲剂对于复发性流产的治疗作用可能是通过上调Ras/MAPK mRNA及蛋白表达实现的。Objective:To investigate the effect of Bushen Antai Granule on the mRNA and protein expression of ras protein/mitogen activated protein kinase mediated by vascular endothelial growth factor receptor 2 with small interference RNA interference.Methods:This study has constructed the placenta microvascular endothelial cells,prepared kidney fetus granule drug-containing serum,and then selected the best drug-containing serum concentration,which has been divided into normal group,the se-rum siRNA-NC normal serum group,drug serum group,siRNA normal serum group,and siRNA drug serum group,using realtime fluorescent quantitative PCR,Western blotting,immunofluorescence test to detect respectively the Ras/MAPK mRNA and protein expression.Results:There was no significant difference in Ras and MAPK mRNA and protein expression between the normal group and the negative control group(P>0.05).The mRNA and protein expressions of Ras and MAPK in the drug serum group were significantly higher than those in the normal serum group(P<0.01).Ras and MAPK mRNA and protein expressions significantly decreased in siRNA1 normal serum group compared with normal serum group(P<0.01).Ras,MAPK mRNA and protein expression in siRNA1 drug serum group were significantly different from those in siRNA1 normal serum group(P<0.01).Conclusion:The therapeutic effect of Bushen Antai Granule on recurrent abortion may be realized by upregulation of Ras/MAPK mRNA and protein expression.

关 键 词：补肾安胎冲剂 复发性流产 胎盘微血管内皮细胞 含药血清 Ras/MAPK信号通路 

分 类 号：R285[医药卫生—中药学]