基于网络药理学探究三七治疗玻璃体积血的作用机制  

Mechanism of panax notoginseng in treating vitreous hemorrhage based on network pharmacology

作　　者：韩梦雨 刘自强 农璐琪 王志军[2] 金明[2] HAN Meng-yu;LIU Zi-qiang;NONG Lu-qi;WANG Zhi-jun;JIN Ming(Beijing University of Traditional Chinese Medicine,Beijing 100029,China;China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学 [2]中日友好医院,北京100029

出　　处：《海南医学院学报》2021年第9期695-700,共6页Journal of Hainan Medical University

基　　金：中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者。

摘　　要：目的:探讨三七治疗玻璃体积血(VH)的协同作用机制。方法:采用TCMSP数据库筛选三七的有效成分,并收集其对应的作用靶点;使用GeneCards、OMIM数据库检索筛选与VH相关的基因靶点,并将三七作用靶点与疾病基因进行映射;利用STRING数据库和Cytoscape 3.7.2软件构建蛋白⁃蛋白相互作用网络图和“药物⁃活性成分⁃疾病⁃靶蛋白”相互作用网络,筛选出核心作用靶基因,最后对潜在治疗靶点进行基因本体(GO)生物过程和KEGG的代谢通路富集分析。结果:筛选出三七主要有效成分8种,涉及162种蛋白。VH的疾病靶点有1387个,其中75个是三七治疗VH的潜在靶点。“药物⁃活性成分⁃疾病⁃靶蛋白”相互作用网络中,共包含节点82个,核心作用靶点包括AKT1、CASP3、VEGF⁃A、IL⁃6和MMP⁃9等;通过GO及KEGG富集分析,筛选出与VH主要成分相关的信号通路143条,关键信号通路包括AGE⁃RAGE信号通路、流体剪切应力与动脉粥样硬化等,作用较大的分子功能是细胞因子活性、受体配体活性、细胞因子受体结合等。结论:网络药理学分析提示,三七治疗VH潜在的分子机制与抗血管生成、抗炎症及调控细胞凋亡和氧化应激等生物学过程密切相关,AKT1、CASP3、VEGF⁃A、IL⁃6和MMP⁃9可能是核心作用靶点。Objective:To investigate the mechanism of panax notoginseng in the treatment of vitreous hematoma(VH).Methods:The active components of panax notoginseng were screened by TCMSP,and the corresponding action targets were also collected.GeneCards and OMIM databases were used to search and screen gene targets related to VH,and panax notoginseng action targets were mapped to disease genes.The core genes were visualized and screened by Cytoscape3.7.2 software,and the potential therapeutic targets were analyzed by GO and KEGG pathways.Results:Eight active components of panax notoginseng were screened,involving 162 proteins.There are 1387 disease targets of VH,among which 75 are potential targets of panax notoginseng for the treatment of VH.There are 82 nodes in the drugactivecomponentdiseasetarget protein interaction network.The core target genes include AKT1,CASP3,VEGFA,IL6 and MMP9.Through GO and KEGG enrichment analysis,143 signaling pathways related to the main components of VH were screened out.The key signaling pathways included AGERAGE signaling pathway,fluid shear stress and atherosclerosis,etc.The molecular functions that played a significant role were cytokine activity,receptor ligand activity,and cytokine receptor binding,etc.Conclusion:The network pharmacology analysis suggests that the potential molecular mechanism of panax notoginseng in the treatment of VH is closely related to the intervention of oxidative stress,angiogenesis,inflammation,apoptosis and extracellular matrix degradation.AKT1,CASP3,VEGFA,IL6 and MMP9 may be the core target genes.

关 键 词：三七 玻璃体积血 网络药理学 药理机制 

分 类 号：R285.5[医药卫生—中药学]