蜂王浆对雌性未孕大鼠乳腺组织的影响  

Effects of royal jelly on mammary gland tissue of non-pregnant female rats

作　　者：刘一冰 陈恒 徐浩 周桂华 蔺哲广 吉挺[1] LIU Yibing;CHEN Heng;XU Hao;ZHOU Guihua;LIN Zheguang;JI Ting(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Guangxi Honey Bee Industry Co.LTD,Nanning 530000)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]广西蜜博士蜂业有限责任公司,南宁530000

出　　处：《中国实验动物学报》2021年第2期160-167,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(31902220);国家蜂产业技术体系专项(CARS-45-SYZ6);江苏省科技支撑计划(BE2018353)。

摘　　要：目的本研究旨在探讨蜂王浆(Royal Jelly,RJ)对雌性未孕大鼠(青春期末到性成熟初期)血清激素水平和乳腺组织的影响。方法将50只Wistar大鼠随机分为5组,每组10只。处理组大鼠每只灌服不同浓度(100、200、400、800 mg/(kg·d))RJ,对照组(CK组)大鼠每只灌服无菌水(每天2 mL)。35 d后,测定大鼠生长指标和乳头直径;运用酶联免疫吸附测定法测定大鼠血清雌激素(E2)、孕激素(P)、催乳素(PRL)水平;对乳腺组织进行苏木精-伊红染色后,观察其组织学形态变化。结果RJ200组和RJ400组大鼠血清E2、PRL水平显著高于CK组(P<0.05),而RJ200、RJ400和RJ800组血清P水平显著低于CK组(P<0.05),RJ100组与CK组血清E2、P、PRL水平均不显著(P<0.05)。乳腺导管直径结果显示,与CK组相比,各处理组导管直径均显著升高(P<0.05),其中RJ100组<RJ200、RJ400和RJ800组,乳腺组织学变化与导管直径结果相一致。另外,RJ200和RJ800组大鼠乳头直径较CK组略有增加(P<0.05)。结论每日摄入低剂量(100 mg/kg)RJ不会对雌性未孕大鼠血清激素水平和乳腺发育造成影响。但当摄入量达到200 mg/(kg·d)或400 mg/(kg·d)时,可能对雌性未孕大鼠乳腺组织的发育和血清激素水平的稳定带来不利影响。Objective The purpose of this study was to investigate the effect of royal jelly(RJ)on serum hormone levels and mammary gland tissues of female non-pregnant rats(late puberty to early sexual maturity).Methods Fifty Wistar rats were randomly divided into 5 groups with 10 rats in each group.Rats in the treatment group were given RJ at different doses(100,200,400,800 mg/(kg·d)),while rats in the control group(CK group)were given sterile water(2 mL).After 35 d,the growth index and nipple diameter were measured.The serum levels of estrogen(E2),progesterone(P)and prolactin(PRL)were measured by ELISA.After hematoxylin-eosin staining of breast tissue,histological morphological changes were observed.Results The result showed that serum E2 and PRL levels in the RJ200 group and RJ400 group were significantly higher than those in the CK group(P<0.05),while serum P levels in the RJ200,RJ400 and RJ800 groups were significantly lower than those in the CK group(P<0.05).Serum E2,P and PRL levels in the RJ100 and CK groups were not significantly different(P<0.05).Breast duct diameter measurements showed that compared with the CK group,duct diameter in each of the treatment groups was significantly increased(P<0.05).The histological changes in the breast were consistent with duct diameter in the RJ100 group being lower than that in the RJ200,RJ400 and RJ800 groups.In addition,the diameter of the nipple in the RJ200 and RJ800 groups was slightly increased compared with the CK group(P<0.05).Conclusions Daily intake of low-dose(100 mg/kg)RJ did not affect serum hormone levels or breast development in female non-pregnant rats.However,at higher doses of 200 and 400 mg/(kg·d),it may adversely affect the development of breast tissue and the stability of serum hormone levels in female nonpregnant rats.

关 键 词：蜂王浆 大鼠 雌性 乳腺 血清激素 

分 类 号：Q95-33[生物学—动物学]