山东部分地区猪圆环病毒2型遗传变异分析  

Genetic variation analysis of porcine circovirus type 2 strains in Shandong province

作　　者：薛瑞雪 田野[2] 杨婉婷 王贵升[1] 马慧玲[1] 李玉杰[1] 王苗利[1] 蔺晓月 陈峰 孙圣福[1] 李云岗[1] XUE Rui-xue;TIAN Ye;YANG Wan-ting;WANG Gui-sheng;MA Hui-ling;LI Yu-jie;WANG Miao-li;LIN Xiao-yue;CHEN Feng;SUN Sheng-fu;LI Yun-gang(Shandong Provincial Center for Animal Control and Prevention,Jinan 250010,China;College of Veterinary Medicine,China Agricultural University,Beijing 100833,China;College of Veterinary Medicine,Shandong Agricultural University,Tai'an 271018,China)

机构地区：[1]山东省动物疫病预防与控制中心,山东济南250010 [2]中国农业大学兽医学院,北京100833 [3]山东农业大学动物医学学院,山东泰安271018

出　　处：《中国预防兽医学报》2021年第2期198-201,218,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家重点研发计划项目(2018YFD0500800)。

摘　　要：为了解山东地区猪圆环病毒2型(PCV2)流行病学特点和遗传变异情况,本研究利用PCR方法对从无害化处理场和屠宰场采集的1477份猪的组织样品进行了PCV2检测和部分PCV2全基因组克隆分析。结果显示,1477份样品中,有122份样品为PCV2阳性,阳性率为8.26%。选取22株进行病毒全基因组扩增,经过序列比对和遗传进化分析显示,22株病毒分别为PCV2a(5株)、PCV2b(7株)、PCV2d(3株)和PCV2e(7株),表明山东地区PCV2流行基因型具有多样性,并且以PCV2b和PCV2e流行为主。与其他基因型相比,PCV2优势基因型PCV2b和PCV2e ORF2的氨基酸位点变化分别集中在^(59)K、^(190)T和^(47)S、^(63)T、^(130)F、^(131)P、^(133)S、^(197)I、^(191)K位点,其中优势基因型PCV2e与LG、DBN-SX07-2、SH和ZJ这4株疫苗株的Cap蛋白抗原指数差异最大,提示了疫苗可能对山东省当前流行的PCV2免疫效果不理想。本研究结果可为后续PCV2分子生物学研究及疫苗的选用提供参考依据。In order to investigate molecular epidemiology and genetic variation of porcine circovirus type 2(PCV2),a total of 1477 samples were collected from harmless disposal field and slaughter houses and then subjected to PCV2 detection.The results showed that the positive rate of PCV2 was 8.26%(122/1477).Twenty-two PCV2 positive samples were selected for complete genome sequence analysis.Multiple sequence alignment in phylogenetic analysis results showed that 5 of 22 PCV2 positive samples belonged to PCV2a subgenotype,7 of 22 belonged to PCV2b subgenotype,3 of 22 belonged to PCV2d subgenotype,and 7 of 22 belonged to PCV2e subgenotype.These data indicate that the genetic diversity of PCV2 was complex in Shandong province,and the dominant subgenotype was PCV2b and PCV2e.Based on the analysis of ORF2 acid amino sequences,the different sites of PCV2b and PCV2e mainly located at ^(59)K,^(190)T and ^(47)S,^(63)T,^(130)F,^(131)P,^(133)S,^(197)I,and ^(191)K,respectively.The antigenic index of PCV2e Cap protein,contains the largest difference compared with that of PCV2 vaccine strains LG,DBN-SX07-2,SH and ZJ,indicating that the PCV2 vaccine may not be effective against currently prevalent PCV2 strains in Shandong.The results can provide the reference for PCV2 molecular biology analysis and vaccine selection in future.

关 键 词：猪圆环病毒2型 流行病学调查 ORF2 抗原指数 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]