及己根、茎、叶醇提取物诱导肺毒性的比较研究  

Comparative study on pulmonary toxicity induced by the ethanol extracts of root,stem and leaf from Chloranthus serratus

作　　者：杜云艳 孙淑萍 杨梅[2] 董婷玉 吴成华 朱恩泽 DU Yunyan;SUN Shuping;YANG Mei;DONG Tingyu;WU Chenghua;ZHU Enze(Graduate School,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院研究生学院,安徽芜湖241002 [2]皖南医学院药学院,安徽芜湖241002 [3]皖南医学院天然日化研究所,安徽芜湖241002

出　　处：《皖南医学院学报》2021年第2期115-119,共5页Journal of Wannan Medical College

基　　金：安徽省教育厅高校自然科学研究项目(KJ2013B310);安徽省卫生和计划生育委员会中医药课题(2016zy95);皖南医学院重点科研项目培育基金(WK2014Z03)。

摘　　要：目的:探究及己根、茎、叶醇提取物诱导大鼠肺损伤的程度及机制。方法:将SD大鼠随机等分为正常(KB)组、根醇提取物(GC)组、茎醇提取物(JC)组和叶醇提取物(YC)组。计算大鼠体质量变化率和肺脏指数;观察肺组织病理学变化;检测肺组织中过氧化氢酶(CAT)、丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)含量和细胞间黏附分子-1(ICAM-1)阳性表达率及血红素氧化酶1(HO-1)蛋白表达水平。结果:第15天JC、YC组大鼠体质量增长率较GC组低,且大鼠肺表面出血点较明显。镜下见JC和YC组肺组织较GC组有大量炎细胞浸润、脂肪变态反应及淀粉样变性,且YC组肺间质实质化。与KB和GC组相比,MDA水平、ICAM-1阳性表达的升高和GSH、SOD、CAT水平及HO-1蛋白表达的降低仅在JC和YC组表现明显的统计学意义,且YC组更明显。结论:及己JC、YC提取物能通过氧化应激诱导较严重的大鼠肺损伤;其根醇提取物毒性较小,可作为新型抗炎药物进一步研究。Objective:To investigate the pulmonary toxicity and mechanisms induced by ethanol extracts of Chloranthus serratus(C.serratus)from its roots,stems and leaves.Methods:Sprague-Dawley(SD)rats were randomLy divided into groups of blank(KB),root ethanol extract(GC),stem ethanol extract(JC)and leaf ethanol extract(YC).The body weight of the rats and the lung index were calculated,and the pathological changes of lung tissues were observed.The contents were measured on catalase(CAT),malondialdehyde(MDA),total superoxide dismutase(T-SOD)and glutathione(GSH),positive expression rate of intercellular adhesion molecule-1(ICAM-1)and heme oxygenase-1(HO-1)protein expression level in the lung tissues.Results:The growth rate was lower in rats in JC and YC groups than in those in GC group,and the bleeding points were more obvious on the lung surface of the rats.Microscopical examination revealed greater inflammatory cell infiltration,adipose allergy and amyloidosis in the lung tissues of the rats from JC and YC groups and materialized pulmonary tissues in rats in YC group.Increased MDA level,positive ICAM-1 expression and decreased levels of GSH,SOD,CAT and HO-1 protein expression had significant statistical significances in the JC and YC groups,and the significance was more evident in YC group.Conclusion:YC and JC extracts can induce severe pulmonary toxicity to rats through oxidative stress,and GC extract has the lowest toxicity and can be used as a new anti-inflammatory medicine by further verification.

关 键 词：及己 肺毒性 细胞间黏附分子-1 血红素氧化酶1 氧化应激 

分 类 号：R285.5[医药卫生—中药学] R331.141[医药卫生—中医学]