3种植物提取物对梨叶黑斑病菌抑制作用研究  

Study on the Inhibitory Effect of Three Kinds Plants Extracts on Pear Leaf Black Spot

作　　者：高小宽[1] 张眉 GAO Xiaokuan;ZHANG Mei(Department of Biology,Hengshui College,Hengshui 053000,China)

机构地区：[1]河北省衡水学院生命科学学院,河北衡水053000

出　　处：《果树资源学报》2021年第3期19-23,共5页Journal of Fruit Resources

基　　金：衡水市科技计划研究资助项目(2019014025Z);衡水学院自然科学类项目(编号:2020ZR20)。

摘　　要：以梨黑斑病原菌(Alternaria kikuchiana Tanaka)为供试菌株,采用离体叶盘法、菌丝生长速率测定法和病原孢子萌发率方法,研究了三七、五味子和银杏3种提取物不同浓度对梨叶黑斑病原菌的抗菌活性。结果表明:3种植物提取物在不同的浸泡时间随着处理液浓度的增加抑制率呈现先升高后降低的趋势,3种植物提取物对梨叶黑斑病菌的最佳抑制浓度均为2000 mg/L,三七提取物对梨叶黑斑病抑菌浸泡时间最佳是2 h,而五味子、银杏提取物浸泡时间则为3 h。三七提取物对梨叶黑斑病抑菌效果最好。Taking the pathogen of pear black spot(Alternaria kikuchiana Tanaka)as the trial bacterial strain,using the methods of leaf disc in vitro test,hypha growth ratio test and the pathogen spore germination ratio test,the inhibitory effect of the extracts with different density from panax notoginseng,schisandra and ginkgo biloba was studied.The results showed that the inhibitory effect of the three kinds plants extracts increased first and then decreased with the increase of the concentration of the solution in different soaking time.The optimum inhibitory concentration of the three kinds plants extracts on Pear leaf black spot pathogen was 2000 mg/L.The optimum soaking time of Panax notoginseng extract for the inhibitory effect on pear leaf black spot was 2 h,while the optimum soaking time of schisandra and ginkgo extracts was 3 h.Panax notoginseng extract had the best antibacterial effect on pear leaf black spot.

关 键 词：梨黑斑病 植物提取物 离体叶盘法 

分 类 号：S436.612.14[农业科学—农业昆虫与害虫防治]