玉米转录因子ZmbZIP77基因克隆及表达分析  

Cloning and expression analysis of maize transcription factor ZmbZIP77 gene

作　　者：邹华文[1] 郭凯 张莹莹 侯留迪 朱叶青 胡蓉蓉 周开明 张丙林 刘卫娟 ZHOU Huawen;GUO Kai;ZHANG Yingying;HOU Liudi;ZHU Yeqing;HU Rongrong;ZHOU Kaiming;ZHANG Binglin;LIU Weijuan(College of Agriculture,Yangtze University,Jingzhou 434025,Hubei)

机构地区：[1]长江大学农学院,湖北荆州434025

出　　处：《长江大学学报（自然科学版）》2021年第2期89-94,共6页Journal of Yangtze University(Natural Science Edition)

基　　金：国家自然科学基金项目“锌指蛋白类转录因子ZmLSD1调控玉米耐盐性的分子机理研究”(31971839);“玉米转脂蛋白新成员ZmLTP3的抗盐功能及其上游调控机制研究”(31471510);湖北省国际合作项目“巴基斯坦耐高温玉米种质资源的引进、鉴定及关键基因挖掘”(2017AHB056)。

摘　　要：碱性亮氨酸拉链蛋白(Basic Leucine Zipper,bZIP)是广泛存在于真核生物中的一类转录因子,广泛参与植物多种生理进程。从玉米(Zea mays L.)中克隆出1个碱性亮氨酸拉链蛋白家族成员ZmbZIP77,对该基因进行生物信息学分析,并采用实时荧光定量PCR技术分析了其在玉米不同组织部位及不同胁迫处理下的表达谱。结果表明,ZmbZIP77与其他bZIP家族成员一样,均含有1个保守的GBF1(Plant G-box binding factor 1)结构域,暗示它和该家族中其他成员具有相同或相似的功能;根据bZIP序列的同源性,植物bZIP序列可以分为3类,ZmbZIP77位于第Ⅱ类,与小麦Wlip9a和拟南芥AtbZIP10亲缘关系最近;玉米ZmbZIP77基因在根中表达量最高,胚芽鞘次之,在叶中表达量最低;高温、干旱和脱落酸均能显著诱导玉米根中ZmbZIP77的表达,提示ZmbZIP77可能在玉米组织发育及逆境胁迫响应中起到重要作用。Basic Leucine Zipper(bZIP)protein is one kind of transcription factors widely found in eukaryotes,which are involved in various physiological processes of plants.In this study,ZmbZIP77,a member of the basic leucine zipper protein family,was cloned from maize(Zea Mays L.)and its gene was analyzed via bioinformatic methods.Meanwhile,its expression profiles in different tissue parts of maize and under different stress treatments were analyzed by real-time fluorescence quantitative PCR.The results show that as other bZIP family numbers,ZmbZIP77 contains one conserved GBF1(Plant G-box binding factor 1)domain,indicating that ZmbZIP77 has the same or similar functions;According to the homology of the bZIP sequences,the plant bZIP can be divided three classes.ZmbZIP77 belongs to classⅡ,sharing the highest sequence homologies with wheat Wlip9a and Arabidopsis AtbZIP10;ZmbZIP77 gene has the highest expression level in roots,followed by coleoptiles,and has the lowest expression level in leaves;high temperature,drought and abscisic acid can significantly induce the expression of ZmbZIP77 in maize roots,suggesting that ZmbZIP77 may play an important role in maize tissue development and stress response.

关 键 词：玉米(Zea mays L.) 碱性亮氨酸拉链蛋白 转录因子 非生物胁迫 

分 类 号：Q78[生物学—分子生物学] S513[农业科学—作物学]