三肠目涡虫(Tricladida)中常用的分子标记及研究进展  

Common molecular markers and research progress in Tricladida

作　　者：王磊[1,2] 董自梅 陈广文[1] 刘德增[1] Wang Lei;Dong Zimei;Chen Guangwen;Liu Dezeng(College of Life Sciences,Henan Normal University,Xinxiang 453007,China;Medical College,Xinxiang University,Xinxiang 453003,China)

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]新乡学院医学院,河南新乡453000

出　　处：《河南师范大学学报（自然科学版）》2021年第3期78-84,共7页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(32070427,31570376,31471965,U1604173);河南省重大公益性项目(201300311700).

摘　　要：介绍了三肠目涡虫研究中常用的分子标记,以及这些标记基因在DNA条形码、分子系统发育、遗传多样性、种群历史动态和系统地理学等方面的研究进展,并展望了这些分子标记在三肠目涡虫研究中的应用前景以及研究热点.目前,核糖体18S、28S、ITS-1与线粒体基因COI是三肠目涡虫研究中最常用的标记基因,18S,28S常与COI,ITS-1联合构建序列用于分析种间进化关系.COI,ITS-1作为DNA条形码常用于新物种分类学研究,此外还广泛用于遗传多样性、种群历史动态等相关研究.A number of molecular markers are summarized in this paper.These markers have broad applications in Tricladida in the following areas:DNA bar-coding,molecular phylogeny,genetic diversity analysis,population history,phylogeography,etc.The research prospects and focus of these markers are also forecast in this paper.Presently,nuclear ribosomal marker of 18S,28S and ITS-1,and mitochondrial marker of COI are usually used in the research of Tricladida.The concatenated sequences of the four markers are conspicuously availed in molecular phylogeny.As the DNA bar-coding,COI,ITS-1 are frequently used to support the separate species status of new species and distinguish the asexual specimens.

关 键 词：三肠目涡虫 分子标记 DNA条形码 遗传多样性 分子系统发育 

分 类 号：Q953[生物学—动物学]