CD4+CD25+调节性T细胞与原因不明复发性流产的相关性分析  

Correlation of CD4+CD25+regulatory T cells with unexplained recurrent spontaneous abortion

作　　者：刘峰 李传瑞 Liu Feng;Li Chuanrui(Department of Obstetrics,Zaozhuang Maternal and Child Health Hospital,Zaozhuang 277100,China)

机构地区：[1]山东省枣庄市妇幼保健院产科,277100

出　　处：《中国实用医刊》2021年第5期26-29,共4页Chinese Journal of Practical Medicine

摘　　要：目的分析CD4+CD25+调节性T细胞与原因不明复发性流产的相关性。方法抽取2018年10月到2019年10月枣庄市妇幼保健院收治的原因不明复发性流产患者110例作为研究组,另抽取同期收治的正常行人工流产术的患者80例作为对照组。检测并比较两组患者外周血及蜕膜组织中CD4+CD25+调节性T细胞比例、调节性T细胞特异性调节因子Foxp3和EBi3的mRNA的表达水平、血清炎性因子水平,分析其与原因不明复发性流产的相关性。结果研究组外周血以及蜕膜组织中调节性T细胞比例分别为(4.86±1.13)%、(6.85±1.73)%,低于对照组的(9.31±1.88)%、(11.29±2.04)%,P<0.05。研究组外周血以及蜕膜组织中调节因子Foxp3和EBi3的mRNA表达水平均低于对照组,且外周血中Foxp3和EBi3的mRNA表达水平低于蜕膜组织,P<0.05。研究组血清肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)分别为(9.25±1.67)、(11.47±2.95)、(0.29±0.15)、(7.44±1.35)ng/L,而对照组TNF-α、IL-2、IL-6、IL-10分别为(1.46±0.53)、(5.74±1.06)、(4.58±0.93)、(16.62±4.34)ng/L,研究组TNF-α、IL-2水平高于对照组,而IL-6、IL-10水平低于对照组(P<0.05)。结论调节性T细胞比例、调节性T细胞因子水平可能与维持妊娠有关,原因不明复发性流产与调节性T细胞降低有一定的相关性。Objective To analyze the correlation of cluster of differentiation(CD4)+CD25+regulatory T cells with unexplained recurrent spontaneous abortion(URSA).Methods A total of 110 patients with URSA admitted to Zaozhuang Maternal and Child Health Hospital from October 2018 to October 2019 were selected as the study group,and 80 patients with artificial abortion during the same period were selected as the control group.The proportion of CD4+CD25+regulatory T cells in peripheral blood and decidual tissue,mRNA expression levels of regulatory T cell specific regulatory factors Foxp3 and EBi3,and the serum inflammatory factor levels were detected and compared between the two groups.The correlation between the above factors and URSA was analyzed.Results The proportions of regulatory T cells in the peripheral blood and decidual tissues of the study group were(4.86±1.13)%and(6.85±1.73)%,respectively,lower than the(9.31±1.88)%and(11.29±2.04)%of the control group(P<0.05).The expression level of Foxp3 and EBi3 mRNA in maternal peripheral blood and decidua tissues in the study group were lower than those in the control group,and the expression level of mRNA in Foxp3 and EBi3 in maternal peripheral blood were lower than those in decidua tissues(P<0.05).The serum tumor necrosis factor-α(TNF-α),interleukin-2(IL-2),interleukin-6(IL-6),interleukin-10(IL-10)in the study group were(9.25±1.67)ng/L,(11.47±2.95)ng/L,(0.29±0.15)ng/L,(7.44±1.35)ng/L,respectively,while those in the control group were(1.46±0.53)ng/L,(5.74±1.06)ng/L,(4.58±0.93)ng/L,(16.62±4.34)ng/L,respectively.The levels of TNF-αand IL-2 in the study group were higher than those in the control group,while the levels of IL-6 and IL-10 in the study group were lower than those in the control group(P<0.05).Conclusions The proportion of regulatory T cells and the level of regulatory T cytokines may be related to the maintenance of pregnancy.And URSA is related to the reduction of regulatory T cells.

关 键 词：流产 原因不明 复发 调节性T细胞 

分 类 号：R714.21[医药卫生—妇产科学]