微创获取人脂肪来源干细胞的分离培养和鉴定  

Isolation,culture and identification of human adipose-derived stem cells from minimally invasive method

作　　者：王影 于佃才 罗岸峰 雷华 岑世宏 黄金龙[5] 于登伟 高舒平 WANG Ying;YU Dian-cai;LUO A n-feng;LEI Hua;CEN Shi-hong;HUANG Jin-long;YU Deng-wei;GAO Shu-ping(Henan En-gineering Research Center of Water Environment and Health,Zhengzhou 451150,China)

机构地区：[1]郑州工业应用技术学院河南省水环境与健康工程技术研究中心,河南郑州451150 [2]北京赛托森生物科技发展有限公司,北京100070 [3]首都医科大学基础医学院,北京1O0069 [4]北京雷华医疗美容诊所,北京100144 [5]南京中医药大学附属医院整形外科,江苏南京210029

出　　处：《中国美容整形外科杂志》2021年第4期245-247,I0014,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的探讨一种新型的采用微创技术获取脂肪来制备脂肪来源干细胞(adipose derived stem cells,ADSCs)的方法。方法自2018年5月至2019年8月在北京雷华医疗美容诊所通过腹部穿刺方法获取5例患者腹部脂肪(0.5~1.5 g)组织进行分离培养,扩增ADSCs,并进行干细胞的分化与鉴定分析。结果经过对ADSCs的增殖能力、特异性表面标志物的流式鉴定,成脂分化、成骨分化、软骨分化、血管内皮细胞分化及神经分化的多项分化能力的鉴定,确定了分离出的细胞为脂肪间充质干细胞。结论采用微创技术可以分离获取ADSCs。Objective To explore a new minimally invasive method for isolation of adipose-derived stem cells(ADSCs).Methods From May 2018 to August 2019,0.5 g to 1.5 g abdominal adipose tissues in 5 patients were obtained by puncture needles.After isolation and culture,the differentiation,identification and analysis of ADSCs were conducted.Results After identification of proliferation of ADSCs,flow cytometry analysis,adipocyte differentiation,osteogenic differentiation,chondrogenicd ifferentiation,vascular endothelial cells and neuronal differentiation,the isolated cells were confirmed asadipose mesenchymal stem cells.Conclusion The ADSCs can be isolated and obtained by minimally invasive technology.

关 键 词：脂肪来源干细胞 微创 分离 鉴定 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]