Antrodia Cinnamomea ameliorates neointimal formation by inhibiting infl ammatory cell infi ltration through downregulation of adhesion molecule expression in vitro and in vivo  

作　　者：Yan Zhang Aijin Ma Hao Xi Ning Chen Rong Wang Chenhui Yang Jinbang Chen Pin Lü Fuping Zheng Wenyi Kang 

机构地区：[1]Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University,Beijing 100048,China [2]Cardiovascular Medical Science Center,Department of Cell Biology,Hebei Medical University,Shijiazhuang 050091,China [3]Hebei Food Safety Key Laboratory,Hebei Food Inspection and Research Institute,Shijiazhuang 050091,China [4]National R&D Center for Edible Fungus Processing Technology,Henan University,Kaifeng,Henan 475004,China

出　　处：《Food Science and Human Wellness》2021年第4期421-430,共10页食品科学与人类健康（英文）

基　　金：This work was supported by the National Key Research Project of China(2019YFC1606400);Major Public Welfare Projects in Henan Province(201300110200);National Key Research Project of Hebei Province(20375502D);Natural Science Foundation of Hebei Province(H2019206212);High-level Talent Funding Project of Hebei Province(A201905006);Fund of National R&D Center for Edible Fungus Processing Technology,Henan University(20200109);the Open Fund from Beijing Advanced Innovation Center for Food Nutrition and Human Health(20182025).

摘　　要：The increased vascular infl ammation is a key event in the development of atherosclerotic lesions.Antrodia cinnamomea has been shown to promote anticancerogenic activity through decreasing infl ammation.However,the potential role of A.cinnamomea in cardiovascular diseases remains unexplored.Herein,using carotid arterial ligation models,we found that ethanol extract from A.cinnamomea(EEAC)signifi cantly inhibited neointimal hyperplasia in a dose-dependent manner,accompanied with the reduced expression of activated p65 and infl ammatory cytokines.We also show that EEAC ameliorated TNF-α-induced phosphorylation of p65 and pro-infl ammatory cytokine expression in both vascular smooth muscle cells(VSMCs)and macrophages in vitro.Mechanistically,EEAC suppressed expression levels of intercellular adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule(VCAM-1)in VSMCs,which attenuates the ability of monocytes/macrophages adhesion to VSMCs.Furthermore,the expression level of these adhesion molecules and infi ltration of monocytes/macrophages were also decreased in neointimal VSMCs of arteries pretreated with EEAC.Altogether,our results reveal a novel function of A.cinnamomea in suppressing vascular infl ammation upon ligation injury during neointimal formation,likely through inhibition of infl ammatory cell infi ltration via downregulating the adhesion molecules in VSMCs.Thus,A.cinnamomea may offer a pharmacological therapy to slow down disease progression in patients with vascular injury.

关 键 词：Antrodia cinnamomea Vascular smooth muscle cells Infl ammation Adhesion molecule Neointimal hyperplasia 

分 类 号：TQ460.1[化学工程—制药化工]