Antioxidative and hepatoprotective activities of a novel polysaccharide (LSAP) from Lepista sordida mycelia  

作　　者：Yingyin Xu Yuanhui Li Yuxiao Lu Xiaobin Feng Guoting Tian Qinghong Liu 

机构地区：[1]Soil and Fertilizer Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China [2]Department of Vegetables,College of Horticulture,China Agricultural University,Beijing 100193,China [3]Department of Environment and Chemical Engineering,Tangshan College,Tangshan 063000,China [4]Institute of Biotechnology and Germplasmic Resource,Yunnan Academy of Agricultural Science,Kunming 650223,China

出　　处：《Food Science and Human Wellness》2021年第4期536-544,共9页食品科学与人类健康（英文）

基　　金：This study was supported by grants from the National Key R&D Program of China(Project No.2018YFD0400200);the China Agriculture Research System(No.CARS-20-08B).

摘　　要：A novel alkali-soluble polysaccharide from Lepista sordida(LSAP)mycelia with antioxidative and hepatoprotective activities was characterized.The weight-average molecular weight and number-average molecular weight of LSAP were 1.442×10^(3) and 6.05×10^(2) kDa,respectively.LSAP was consisted of glucose(57.9%),xylose(31.8%),and small amounts of rhamnose,arabinose,galactose,glucuronic acid,and galacturonic acid(1.2%–3.1%).The FT-IR and 2D NMR confi rmed that LSAP was composed of Xylp,Araf,4-O-Me-α-D-GlcpA,(1→4)-linkedβ-D-Glcp,and(1→4)-α-D-GalA,andβ-glycosidic linkages between these sugar units.LSAP displayed notable effects on 1,1-dephenyl-2-picryhydrazyl(DPPH)radical scavenging,hydrogen peroxide scavenging,lipid peroxidation inhibitory ability,reducing power and Fe^(2+)chelating property.These biological effects were further verifi ed by suppressing CCl_(4)-induced oxidative liver damage in mice at doses of 100 and 200 mg/kg.Administration of LSAP in mice prior to CCl_(4) signifi cantly prevented the CCl_(4)-induced elevation in serum alanine aminotransferase,aspartate aminotransferase,and hepatic malondialdehyde.Mice treated with LSAP demonstrated to increase activities in superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the liver.We also found out that LSAP prevented CCl_(4)-induced oxidative liver histological alteration.LSAP may exert hepatoprotective effects against CCl_(4)-induced damage through antioxidant mechanisms in model mice.

关 键 词：Lepista sordida POLYSACCHARIDE Antioxidant activity Hepatoprotective effects 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]