3种梨野生砧木资源叶片抗氧化酶活性分析  

Analysis of antioxidant enzyme activity in the leaves of 3 species of Pyrus as rootstock

作　　者：王超 霍宏亮[1] 田路明[1] 董星光[1] 齐丹[1] 徐家玉 刘超 张思梦 杨祥 郭瑞 曹玉芬[1] WANG Chao;HUO Hongliang;TIAN Luming;DONG Xingguang;QI Dan;XU Jiayu;LIU Chao;ZHANG Simeng;YANG Xiang;GUO Rui;CAO Yufen(Research Institute of Pomology,CAAS,Xingcheng 125100,Liaoning,China)

机构地区：[1]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《果树学报》2021年第5期646-654,共9页Journal of Fruit Science

基　　金：现代农业产业技术体系建设专项(CARS-29-01);中国农业科学院科技创新工程(CAAS-ASTIP)。

摘　　要：[目的]探究杜梨、木梨、秋子梨3种重要梨野生砧木资源抗氧化酶(SOD、POD、CAT)活性差异及盐碱胁迫处理后的变化。[方法]采集大树和实生苗期杜梨、木梨、秋子梨资源叶片,进行盐碱胁迫处理,测定处理前后SOD、POD、CAT活性并比较其差异。[结果]SOD活性,杜梨>秋子梨>木梨,胁迫处理后均明显上升;POD活性,秋子梨>木梨>杜梨,胁迫处理后均显著上升;而CAT活性,木梨>杜梨>秋子梨,处理后仅杜梨活性升高。杜梨叶片抗氧化酶活性强于木梨、秋子梨。山西杜梨1和山西杜梨3耐盐碱性强。[结论]盐碱胁迫处理后,SOD平均活性增加最多,其次为POD平均活性,CAT平均活性降低。杜梨抗氧化酶活性及耐盐碱性强于木梨、秋子梨。[Objective]P.betulaefolia,P.ussuriensis and P.xerophila are the main rootstocks in the North,Northeast and Northwest of China.Antioxidant enzymes have close connection with plant growth and development.The aim of the study was to investigate the antioxidant enzyme(SOD,POD and CAT)activities among 3 species of pyrus and the connection between the antioxidant enzyme(SOD,POD and CAT)activities and the salt resistance.[Methods]Six accessions of each species were collected from"National Repository of Apple and Pear"in Research Institute of Pomology,Chinese Academy of Agricultural Sciences in August 2019.The seeds of 8 accessions were collected and stratified and then sown in the soil for germination.60-day old seedlings were treated with saline-alkali solution.The activities of SOD,POD and CAT of both the leaves from the adult trees of 18 accessions and from the leaves seedlings of 8 accessions were measured at about 25℃through ultraviolet spectrophotometer.The mean value and standard error of antioxidant enzyme activities and relevance were calculated with SPSS 26.0 software,one-way ANOVA was used to analyze the significant differences,and LSD(minimum significance method)was used to test the significant differences among the accessions atα=0.05 level.Origin 2019 b APP was used for principal component analysis.[Results]Among the 18 wild pear accessions,the activity range of SOD was 37.55-206.64 U·mL-1,with the average level121.57 U·mL-1,and the highest SOD activity was detected in the leaves of P.betulaefolia-Wujiazhuang1.The activity range of POD was 32.71-455.60 U·mL-1,with the average level 177.55 U·mL-1,and the highest POD activity was found in P.xerophila-Huangtupo.The CAT activity range was 58.72-237.71 U·mL-1,with the average level 101.41 U·mL-1,and the highest CAT activity was found in P.xerophilaLingkong 1.Among the activities of POD,SOD and CAT of the 18 wild pear accessions,POD was the highest,followed by SOD and CAT in turn.There were significant differences in SOD and POD activities among 18 acce

关 键 词：梨 砧木 抗氧化酶 

分 类 号：S661.2[农业科学—果树学]