蟾酥壳聚糖微球的制备及体外释药性研究  

Study on preparation of Venenum Bufonis Chitosan Microspheres and sustained release characteristics in vitro

作　　者：刘莉 李宗云 何世学 赵越 张春宝 LIU Li;LI Zongyun;HE Shixue;ZHAO Yue;ZHANG Chunbao(Department of Pharmacy,Hengshui People’s Hospital,Hebei Province,Hengshui 053000,China;Department of Clinical Pharmacy,Hengshui People’s Hospital,Hebei Province,Hengshui 053000,China)

机构地区：[1]河北省衡水市人民医院制剂科,河北衡水053000 [2]河北省衡水市人民医院临床药学科,河北衡水053000

出　　处：《中国医药导报》2021年第13期31-34,46,F0003,共6页China Medical Herald

基　　金：河北省中医药管理局科研计划项目(2018545)。

摘　　要：目的制备蟾酥壳聚糖微球,以脱乙酰壳聚糖作为载体,研究蟾酥壳聚糖微球在体外释药的特点。方法通过乳化-交联法制备蟾酥壳聚糖微球,薄层色谱法对其进行成分鉴别,高效液相色谱法用于酯蟾毒配基及华蟾酥毒基的含量检测和微球包封率(系统中包封的药量比系统中包封与未包封的药量)的测定,紫外分光光度法检测吲哚生物碱类的含量。结果制备的微球表面圆滑,平均粒径为9.20μm;3批微球均出现与对照品相应的荧光斑点且阴性无干扰;微球酯蟾毒配基及华蟾酥毒基的总载药量为6.06%,包封率为73.30%,吲哚生物碱类含量为6.59%。结论制备的蟾酥壳聚糖微球具有相当好的缓释效果。Objective To prepare the Venenum Bufonis Chitosan Microspheres with deacetylated chitosan as a carrier and study the characteristics of Venenum Bufonis Chitosan Microspheres sustained release in vitro.Methods The Venenum Bufonis Chitosan Microspheres were prepared by emulsification-crosslinking method and their components were identified by thin layer chromatography.High performance liquid chromatography was used to detect the content of cinobufagin and resibufogenin and to detect the encapsulated efficiency of microspheres(the amount of drug encapsulated in the system divided by total dose of encapsulated and unencapsulated).The ultraviolet spectrophotometer was used to detect the indole alkaloids.Results The prepared microspheres had smooth surface and an average particle size of 9.20μm.The three batches of microspheres showed fluorescence spots corresponding to the control substance and were negative without interference.The total drug loading of cinobufagin and resibufogenin,encapsulation rate and indole alkaloids content of microspheres were 6.06%,73.30%and 6.59%respectively.Conclusion The prepared Venenum Bufonis Chitosan Microspheres have a good sustained release effect.

关 键 词：蟾酥 壳聚糖 微球 乳化-交联法 缓释 

分 类 号：R944[医药卫生—药剂学]