咽炎消合剂对慢性咽炎大鼠模型咽粘膜TGF-β1、Smad3调节机制研究  

Study on Regulating Mechanism of Yanyanxiao Mixture on Pharyngeal Mucosa TGF-β1 and Smad3 in Chronic Pharyngitis Rat Model

作　　者：李在望 周家璇 黄春江 王艺 LI Zaiwang;ZHOU Jiaxuan;HUANG Chunjiang;WANG Yi(The Second Clinical Medical College of Jinan University/The First Affiliated Hospital of Southern University of Science and Technology/Department of Neurology,Shenzhen People's Hospital,Shenzhen 518020,China;The First Clinical Medical College of Yunnan Uinversity of Chinese Medicine,Kunming 650021,China)

机构地区：[1]暨南大学第二临床医学院/南方科技大学第一附属医院/深圳市人民医院神经内科,广东深圳518020 [2]云南中医药大学第一临床医学院,云南昆明650021

出　　处：《云南中医学院学报》2020年第6期1-5,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81760881)。

摘　　要：目的通过研究咽炎消合剂对慢性咽炎大鼠模型咽部粘膜组织中TGF-β1、Smad3 mRNA表达的趋势,阐明咽炎消合剂修复慢性咽炎模型大鼠咽粘膜的作用机制,进一步研究该病的发病机理。方法选用45只SD大鼠,随机分3组造模:标准慢性咽炎模型组,慢性咽炎模型治疗组,空白对照组。成功造模后进行咽炎消合剂治疗15d,然后对各实验组大鼠粘膜组织病理切片免疫组化检测,以及用Q-PCR检测不同组别中的TGF-β1、Smad3 mRNA的表达情况,对Q-PCR中Ct值进行t检验,按照2^(-ΔΔCt)方法进行计算。结果一般观测及病理检查显示,慢性咽炎模型建立成功。治疗组比较标准慢性咽炎模型组,咽粘膜组织病理增生情况好转明显,局部炎性细胞的数量减少,血管通透性明显降低。Q-PCR检测显示,模型组及治疗组的TGF-β1、Smad3 mRNA表达要高于空白组(P<0.05),经咽炎消合剂治疗后,治疗组TGF-β1、Smad3 mRNA表达明显低于模型组。结论咽炎消合剂对慢性咽炎大鼠模型咽粘膜中有TGF-β1/Smad3信号通路具有调控作用,干预了咽粘膜的组织纤维增生及炎性改变,证实了该药物对慢性咽炎治疗的有效性。Objective By studying the trend of Yanyanxiao Mixture on the expression of TGF-β1 protein in the pharyngeal tissue of chronic pharyngitis(CP)rat models,to clarify and further study the mechanism of Yanyanxiao Mixture repairing the mucosa of CP model rats and the pathogenesis of CP.Methods Forty-five SD rats were selected and randomly divided into 3 groups:standard CP model group,treatment group,and blank control group.After successful model established,treatment by Yanyanxiao Mixture for 15 days,immunohistochemical detection of mucosal tissue pathological sections of rats in each group,and Q-PCR detection of TGF-β1 and Smad3 mRNA expression in different groups.Results Pathological examination showed that compared with the standard CP model group and the treatment group,the number of local pharyngeal mucosal inflammatory cells was reduced,and the vascular permeability was significantly reduced.The expression of TGF-β1 and Smad3 mRNA in the model group and the treatment group was higher than that of the blank group.After treatment with“Yanyanxiao Mixture”,The expression of TGF-β1 and Smad3 mRNA in the treatment group was significantly lower than that in the model group.Conclusion Yanyanxiao Mixture has a regulatory effect on the TGF-β1/Smad3 signaling pathway in the pharyngeal mucosa of rat model of CP,and could interfered the tissue fibrosis and inflammatory changes of the pharyngeal mucosa,confirmed the effectiveness of the Mixture in the treatment of CP.

关 键 词：慢性咽炎 TGF-Β1 SMAD3 动物模型 咽黏膜 咽炎消合剂 

分 类 号：R285.5[医药卫生—中药学]