清支止咳汤联合阿奇霉素对肺炎支原体感染小鼠肺和大肠组织中SP-A及s B7-H3表达的影响  

Effects of Qingzhi Zhike Decoction combined with azithromycin on the expression of SP-A and sB7-H3 in the lung and large intestine of mice infected with mycoplasma pneumonia

作　　者：郝欧美[1] 王雪峰[1] 岳志军[1] 南春红[1] 于艳[1] 张童[2] 刘玉凤[3] 赵雪[1] HAO Ou-mei;WANG Xue-feng;YUE Zhi-jun;NAN Chun-hong;YU Yan;ZHANG Tong;LIU Yu-feng;ZHAO Xue(Affiliated Hospital of Liaoning University Traditional Chinese Medicine,Shenyang 110032,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;The Fourth Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110101,China)

机构地区：[1]辽宁中医药大学附属医院,沈阳110032 [2]辽宁中医药大学,沈阳110032 [3]辽宁中医药大学第四附属医院,沈阳110101

出　　处：《中华中医药杂志》2021年第4期1907-1912,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81774363);辽宁省教育厅基金项目(No.11020138170112)。

摘　　要：目的:通过观察肺炎支原体(MP)感染小鼠肺、肠组织中肺表面活性蛋白A(SP-A)、可溶性B7-H3(sB7-H3)的表达,探讨MP诱导的小鼠肺损伤的机制及中药的干预作用。方法:采用MP菌液滴鼻的方法建立MP诱导小鼠感染模型。将造模成功的50只小鼠分为正常组、模型组、中药组(清支止咳汤)、西药组(阿奇霉素)和中西结合组(清支止咳汤+阿奇霉素),每组10只,清支止咳汤连续干预7d,阿奇霉素连续干预3d,各组均于第8天取材,采用免疫组化和RT-PCR的方法测定肺和肠组织中的SP-A、sB7-H3蛋白及mRNA的表达。结果:与正常组比较,模型组肺组织可见明显肺间质炎症;肠组织可见炎症细胞浸润。模型组中肺组织SP-A表达显著低于正常组(P<0.01);肺、肠组织sB7-H3及肠组织SP-A的表达显著高于正常组(P<0.01,P<0.05)。与模型组比较,各治疗组肺组织SP-A的表达均显著升高(P<0.01,P<0.05),sB7-H3表达显著降低(P<0.05);中药组和中西结合组肠组织SP-A、sB7-H3的表达显著下调(P<0.01,P<0.05),且中西结合组肺、肠组织中SP-A及肠组织中sB7-H3表达改善优于西药组(P<0.01,P<0.05)。结论:清支止咳汤联合阿奇霉素能有效促进肺组织中SP-A的表达,抑制肺、肠组织中sB7-H3及肠组织SP-A的表达,改善MP感染小鼠的肺、肠组织损伤。Objective:To explore the mechanism of lung injury in the mice infected by mycoplasmal pneumonia and provide experimental evidence for clinical treatment by observing the changes of surfactant protein A(SP-A)and B7 homolog 3 protein(sB7-H3)expressions in the lung and the intestines of the mice.The mouse model was established using the MP bacterial droplet nasal method.Fifty female BALB/c mice were randomly divided into 5 groups:control,model,Qingzhi Zhike Decoction group,western medicine group and Chinese and Western combination group,10 mice in each group.Qingzhi Zhike Decoction was administered by oral gavage every day for seven days,and azithromycin was administered for three days,the lung and intestines tissues were collected after 7 days to observe the inflammation of the tissues.The levels of SP-A and sB7-H3 expressions in lung and intestines tissues were assessed by immunohistochemistry staining and RT-PCR.Results:The pathological results showed that compared with the control group,the lung tissue showed obvious pulmonary interstitial inflammation;the intestinal tissue showed inflammatory cell infiltration.The expressions of SP-A in the lung tissue were significantly lower(P<0.01),while the s B7-H3 in lung and intestine tissues and SP-A in the intestine tissue were significantly higher in the model group than the control group(P<0.01,P<0.05).Compared with the model group,the expression of SP-A in the lung tissue in Qingzhi Zhike Decoction group,western medicine group and Chinese and Western combination group were significantly increased(P<0.01,P<0.05),and s B7-H3 in lung tissue were decreased(P<0.05);while the expression of SP-A and sB7-H3 in the intestinal tissue in Qingzhi Zhike Decoction group and Chinese and Western medicine group were significantly decreased(P<0.01,P<0.05).The improvement of SP-A in the lung and intestine tissues and sB7-H3 in the intestinal tissue in Chinese and Western medicine group were better than those in the western medicine group(P<0.01,P<0.05).Conclusion:Qingzhi Zhike Decoctio

关 键 词：肺炎支原体 肺表面活性蛋白A 可溶性B7-H3 肺与大肠相表里 清支止咳汤 阿奇霉素 

分 类 号：R965[医药卫生—药理学]