基于肠道L细胞葡萄糖转运体表达的小檗碱降糖机制研究  

Study on hypoglycemic mechanism of berberine through glucose transporter expression in intestinal L cells

作　　者：杨欣妤 黄明月 时正媛 鄢丹 YANG Xin-yu;HUANG Ming-yue;SHI Zheng-yuan;YAN Dan(Beijing Key Laboratory of Bio-Characteristic Profiling for Evaluation of Rational Drug Use,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;Chengdu University of TCM,Chengdu 610075,China)

机构地区：[1]首都医科大学附属北京世纪坛医院临床合理用药评价北京重点实验室,北京100038 [2]成都中医药大学,成都610075

出　　处：《中华中医药杂志》2021年第4期1926-1930,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市自然科学基金项目(No.7194280);国家自然科学基金项目(No.82004011);国家“重大新药创制”科技重大专项(No.2017ZX09301-040);首都医科大学附属北京世纪坛医院院青年基金项目(No.2016-q17)。

摘　　要：目的:基于肠道L细胞的葡萄糖转运体表达调控探讨小檗碱降糖机制。方法:以CCK8法及实时无标记细胞分析技术(RTCA)测定小檗碱对STC-1细胞活性的影响。ELISA及葡萄糖氧化酶(GOD-POD)法测定小檗碱对STC-1细胞胰高血糖素样肽-1(GLP-1)分泌及葡萄糖消耗的影响,利用qRT-PCR及Western blot技术分析细胞中钠/葡萄糖协同转运蛋白1(SGLT1)、葡萄糖转运蛋白2(GLUT2)mRNA及蛋白表达情况。结果:与正常组比较,5、10、50μmol/L小檗碱显著促进STC-1细胞分泌GLP-1(P<0.01,P<0.05)。10、50μmol/L小檗碱促进葡萄糖的消耗(P<0.01),上调STC-1细胞的SGLT1 mRNA表达(P<0.05),50μmol/L小檗碱上调SGLT1蛋白表达(P<0.05),而各浓度小檗碱对STC-1细胞的GLUT2 mRNA和蛋白的表达量无显著影响。结论:小檗碱促进STC-1细胞GLP-1分泌、提高糖消耗,其作用机制可能与上调SGLT1的表达有关。Objective:To explore the hypoglycemic mechanism of berberine based on the regulation of glucose transporter expression in intestinal L cells.Methods:The effects of berberine on the activity in STC-1 cells were determined by CCK8 and real-time cell analysis(RTCA).The effect of different concentration berberine on glucose consumption of STC-1 cells by glucose oxidase(GOD-POD)method and the level of GLP-1 was tested by ELISA.Western blot and qRT-PCR were used to evaluate the mRNA and protein expression of SGLT1 and GLUT2 in STC-1 cells.Results:Compared with the normal group,5,10,50μmol/L berberine improved the secretion of GLP-1 in STC-1 cells(P<0.01,P<0.05).10,50μmol/L berberine increased the consumption of glucose(P<0.01)and up-regulated the mRNA expression level of SGLT1(P<0.05).50μmol/L berberine upregulated the protein level of SGLT1(P<0.05).However,berberine at different concentrations has no significant effect on the upregulation of GLUT2 mRNA and GLUT2 protein expression in STC-1 cells.Conclusion:Berberine increase the GLP-1 secretion and glucose consumption,which mechanism may be related to the up-regulation of SGLT1 expression.

关 键 词：小檗碱 肠道L细胞 胰高血糖素样肽-1 葡萄糖转运体 

分 类 号：R285[医药卫生—中药学]