雷公藤甲素脂质体的制备及对蛙上颚黏膜纤毛毒性研究  

Study on preparation of triptolide liposomes and its toxicity to maxillary cilia of frog

作　　者：张岚 闫敏[2] 谭晓柯 陈坤 严银银 张振强[2] 朱鑫[1] 宋军营[2] 谢治深 张紫娟[2] 武香香[2] 曾华辉[2] ZHANG Lan;YAN Min;TAN Xiao-ke;CHEN Kun;YAN Yin-yin;ZHANG Zhen-qiang;ZHU Xin;SONG Jun-ying;XIE Zhi-shen;ZHANG Zi-juan;WU Xiang-xiang;ZENG Hua-hui(Pharmacy College,Henan University of Chinese Medicine,Zhengzhou 450046,China;Academy of Chinese Medicine Sciences,Henan University of Chinese Medicine,Zhengzhou 450046,China;School of Basic Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,郑州450046 [2]河南中医药大学中医药科学院,郑州450046 [3]河南中医药大学基础医学院,郑州450046

出　　处：《中华中医药杂志》2021年第4期2334-2337,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.U1604185);河南省科技攻关项目(No.182102410012,No.192102310440);河南省高校科技创新人才支持计划(No.20HASTIT050);河南省高等学校重点科研项目(No.19A360021,No.19A360002,No.20B350003);2020年河南中医药大学科研苗圃工程项目(No.MP2020-13)。

摘　　要：目的:制备雷公藤甲素脂质体,并考察其对蛙上颚黏膜纤毛的粘附性和毒性。方法:采用薄膜分散法制备雷公藤甲素脂质体,并对其进行质量评估。以在体蛙上颚黏膜为模型,研究不同给药剂量下雷公藤甲素及其脂质体对蛙上颚黏膜纤毛的影响,评价脂质体药物的毒性及粘附性。结果:制备的雷公藤甲素脂质体在电镜下呈圆粒状且分布均匀,平均粒径(90.04±7.36)nm,包封率为(62.34±7.29)%;相较溶剂对照组,雷公藤甲素中、高剂量组与雷公藤甲素脂质体高剂量组的纤毛持续运动及转运速率显著下降(P<0.01);脂质体组相较雷公藤甲素原药各剂量组的纤毛持续运动时间显著延长(P<0.01),且显微镜下纤毛排列更整齐活跃。结论:在完善雷公藤甲素脂质体制备工艺条件下,雷公藤甲素脂质体比原药对纤毛损伤小,且粘附性好,能起到明显减毒增效作用。Objective:To prepare triptolide liposome and investigate its adhesion and toxicity to the mucociliary of maxillary mucosa of frog.Methods:Liposomes of triptolide was prepared by thin-film dispersion method and its quality was evaluated.To study the effects of triptolide and its liposomes on the mucociliary of frog’s maxilla,and to evaluate the toxicity and adhesion of the liposomes.Results:The prepared liposome of Tripterygium wilfordii was round and evenly distributed under electron microscope,with an average particle size(90.04±7.36)nm,encapsulation efficiency(62.34±7.29)%;Compared with the solvent control group,the continuous movement and transport rate of cilia in middle and high dose triptolide group and high dose triptolide liposome group decreased significantly(P<0.01);Compared with each dose of triptolide,the duration of cilia in liposome group was significantly prolonged(P<0.01),and the cilia arrangement was more neat and active under microscope.Conclusion:Under the condition of improving the preparation process of triptolide liposome,triptolide liposome has less damage to cilia than the original drug,and has good adhesion,which can obviously reduce toxicity and increase efficiency.

关 键 词：雷公藤甲素 脂质体 纤毛毒性 粘附性 

分 类 号：R285.5[医药卫生—中药学]