微宇宙体系中锑形态及土壤微生物群落结构变化研究  

Changes of Antimony Forms and Soil Microbial Community Structure in Microcosm

作　　者：张文迈 梁震 ZHANG Wen-mai;LIANG Zhen(School of Chemistry and Environmental Engineering,Wuhan Institute of Technology,Hubei Wuhan 430205,China)

机构地区：[1]武汉工程大学化学与环境工程学院,湖北武汉430205

出　　处：《广州化工》2021年第9期92-94,共3页GuangZhou Chemical Industry

摘　　要：采用贵州“喀斯特”地貌中受独山锑矿污染的稻田土样品,构建了实验室尺度的微宇宙体系,结合土壤中总DNA的提取和高通量测序结果,研究培养期内锑形态变化与微生物菌种群落数量变化的关系。结果显示,微生物可以通过氧化三价锑来获取能量,同时减轻锑的毒性,培养期间第5~10 d内Sb(Ⅲ)可完全转化为Sb(Ⅴ);其中根据绝对丰度筛选出的6种门水平上微生物和20种属水平上微生物均为厌氧条件下锑自养氧化细菌。A laboratory scale microcosm system was constructed by using paddy soil samples polluted by Dushan antimony mine in Guizhou karst landform.The relationship between the changes of antimony speciation and the number of microbial communities during the incubation period was studied by combining the results of total DNA extraction and high-throughput sequencing.The results showed that microorganisms can obtain energy by oxidizing trivalent antimony and reduce the toxicity of antimony.Sb(Ⅲ)can be completely converted into Sb(Ⅴ)within 5~10 days of culture.Among them,according to the absolute abundance,6 kinds of microorganisms at the phylum level and 20 kinds of microorganisms at the genus level were antimony autotrophic oxidizing bacteria under anaerobic conditions.

关 键 词：锑污染稻田土 微宇宙体系 锑形态 微生物种群 

分 类 号：X53[环境科学与工程—环境工程]