Temporal variation and sharing of antibiotic resistance genes between water and wild fish gut in a peri-urban river  

作　　者：Zhen-Chao Zhou Ze-Jun Lin Xin-Yi Shuai Ji Zheng Ling-Xuan Meng Lin Zhu Yu-Jie Sun Wei-Chun Shang Hong Chen 

机构地区：[1]Institute of Environmental Technology,College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,China [2]Ningbo Research Institute of Ecological and Environmental Sciences,Ningbo 315012,China

出　　处：《Journal of Environmental Sciences》2021年第5期12-19,共8页环境科学学报（英文版）

基　　金：supported by the Natural Science Foundation of China (Nos. 21876147 and 21677121)。

摘　　要：Antibiotic resistance genes(ARGs) as emergence contaminations have spread widely in the water environment. Wild fish may be recipients and communicators of ARGs in the water environment, however, the distribution and transmission of ARGs in the wild fish and relevant water environment were rarely reported. Here, we have profiled ARGs and bacterial communities in wild freshwater fish and relevant water in a peri-urban river using highthroughput qPCR and 16 S rRNA gene sequence. A total of 80 and 220 unique ARG subtypes were identified in fish and water samples. Fish and water both showed significant ARG seasonal variations( P < 0.05). The highest absolute abundance of ARGs in fish and water occurred in summer(1.32 × 10^(9) copies per g, on average) and autumn(9.04 × 10^(6) copies per mL), respectively. In addition, the bipartite network analysis showed that 9 ARGs and 1 mobile genetic element continuously shared in fish and water. Furthermore, bacteria shared in fish and water were found to significantly correlate with shard ARGs. The findings demonstrate that bacteria and ARGs in fish and water could interconnect and ARGs might transfer between fish and water using bacteria as a spreading medium.

关 键 词：Antibiotic resistance genes Seasonal variation Wild fish Water environment 

分 类 号：X52[环境科学与工程—环境工程] X503.225