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作 者:李清雪[1] 陈景伟[2] 杨剑 LI Qing-xue;CHEN Jing-wei;YANG Jian(Department of Gynecology,the Fourth Hospital of Shijiazhuang City,Shijiazhuang 050011,Hebei Province,China;Department of Obstetrics and Gynecology Integrated with Traditional Chinese and Western Medicine,Hebei College of Traditional Chinese Medicine,Shijiazhuang 050200,Hebei Province,China;Department of Rehabilitation,Hospital of Special Care of Hebei Province,Shijiazhuang 050051,Hebei Province,China)
机构地区:[1]石家庄市第四医院妇一科,河北石家庄050011 [2]河北中医学院中西医结合妇产科,河北石家庄050200 [3]河北省优抚医院康复科,河北石家庄050051
出 处:《中国临床药理学杂志》2021年第8期1004-1007,共4页The Chinese Journal of Clinical Pharmacology
摘 要:目的探讨OTUD7B在子宫内膜异位症中的作用及其机制。方法将子宫内膜基质细胞随机分为四组,分别转染OTUD7B过表达质粒(pcDNA-OTUD7B组)、空白质粒(NC组)、针对OTUD7B的小干扰RNA(si-OTUD7B组)及无意义小干扰RNA(si-NC组)。用荧光定量逆转录-聚合酶链式反应(RT-qPCR)检测细胞OTUD7B mRNA的表达。用CCK-8实验检测细胞增殖水平,用流式细胞术检测细胞凋亡率,用Transwell实验检测细胞侵袭能力。结果OTUD7B mRNA在子宫内膜异位症中表达水平0.31±0.06显著低于正常子宫内膜组织1.03±0.31,差异有统计学意义(P<0.05)。96 h,pcDNA-OTUD7B组、NC组、si-OTUD7B组及si-NC组细胞吸光度分别为0.63±0.07,0.81±0.06,0.93±0.02,0.80±0.07;细胞凋亡率分别为(12.31±1.40)%,(4.17±0.51)%,(1.82±0.20)%和(4.42±0.40)%;侵袭细胞数目为(19.67±2.52),(59.67±6.02),(96.67±10.78)和(51.33±5.03)个,pcDNA-OTUD7B组与NC组比较,si-OTUD7B组与si-NC组比较,差异有统计学意义(P<0.05)。结论OTUD7B通过抑制NF-κB信号通路抑制子宫内膜基质细胞的增殖和侵袭,并促进细胞凋亡。Objective To explore the role and mechanism of OTUD7 B in endometriosis.Methods The endometrial stromal cells were randomly divided into four groups which were transfected with OTUD7 B overexpression plasmid(pcDNA-OTUD7 B group),blank plasmid(NC group),small interference RNA against OTUD7 B(si-OTUD7 B group)and meaningless small interference RNA(si-NC group).The quantitative reverse transcriptase polymerase chain reaction(RT-qPCR)was used to detect cell OTUD7B mRNA expression.CCK-8 assay was used to detect cell proliferation.Flow cytometry was used to detect apoptosis.Transwell assay was used to detect cell invasion.Results OTUD7B mRNA was significantly lower in endometriosis(0.31±0.06)than normal endometrial tissue(1.03±0.31).The difference was statistically significant(P<0.05).At 96 h,the absorbance of pcDNA-OTUD7 B group,NC group,si-OTUD7 B group and si-NC group were 0.63±0.07,0.81±0.06,0.93±0.02,0.80±0.07;the apoptosis rates of each group after 48 h culture was(12.31±1.40)%,(4.17±0.51)%,(1.82±0.20)%and(4.42±0.40)%;the invasion cell numbers were19.67±2.52,59.67±6.02,96.67±10.78 and 51.33±5.03.Compared between pcDNA-OTUD7 B group and NC group,compared between si-OTUD7 B group and si-NC group,difference was statistically significant(P<0.05).Conclusion OTUD7 B inhibits the cell proliferation and invasion,and promotes cell apoptosis in endometriosis by inhibiting the NF-κB signaling pathway.
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