中药植物羌活种子休眠解除的代谢组分析  

Metabolomics Analysis of Seed Dormancy Release of Traditional Chinese Medicine Plant Notopterygium incisum

作　　者：李爱花 蒋顺媛 郭娜[4] 黄璐琦[2] LI Aihua;JIANG Shunyuan;GUO Na;HUANG Luqi(Beijing Floriculture Engineering Technology Research Center,Beijing Botanical Garden,Beijing 100093,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Sichuan Key Laboratory of Quality and Innovation Research of Chinese Materia Medica,Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]北京市植物园,北京市花卉园艺工程技术研究中心,北京100093 [2]中国中医科学院中药资源中心,国家道地药材重点实验室培育基地,北京100700 [3]四川省中药科学研究院,四川省中药质量和创新研究重点实验室,成都610041 [4]中国中医科学院实验研究中心,北京100700

出　　处：《中国农业科技导报》2021年第5期44-51,共8页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(81325023);中国博士后基金项目(ZZ2016012);北京市植物园园管课题项目(BZ202103)。

摘　　要：传统中药植物羌活的种子因具有休眠限制了其人工驯化和栽培种植。为了解析羌活种子休眠解除过程的代谢物变化,筛选休眠解除关键化合物,对羌活种子进行冷暖层积预处理150 d,并对预处理的种子进行低温层积处理110 d,以及ABA抑制剂氯定酮(FL)和GA_(3)(GA)处理,用UPLC-MS/MS对处理过程的羌活种子进行代谢物分析。结果表明:羌活种子整个处理过程分为3个阶段,0—60 d、90—(150+30)d、(150+60)—(150+110)d。其中,预处理+低温层积处理(150+60)d和(150+110)d的羌活种子已具备萌发能力。t[1]主成分所代表的化合物是预处理0、15、60、90、120 d的胚发育处理过程发生主要变化的化合物。t[2]主成分所代表的化合物主要在生理休眠解除过程发生变化。差异分析发现,列当醇乙酸酯是一种独脚金内酯化合物,在种子休眠解除过程发生显著积累。其中,FL处理后列当醇乙酸酯的生物合成水平升高12.1倍,而低温层积处理后其合成水平升高28.5倍。因此,推测列当醇乙酸酯含量变化对羌活种子休眠解除非常关键。研究结果可为羌活种子休眠解除机理研究提供理论基础,对中药羌活的产业发展具有重要的实践意义。The seeds of Notopterygium incisum,a traditional Chinese medicine plant,are dormant,which limits its artificial domestication and cultivation.In order to analyze the metabolite changes and screen the key compounds during releasing dormancy of N.incisum seeds,seeds of N.incisum were pretreated with cold and warm stratification;and the pretreated seeds were treated with low temperature stratification(CS),ABA inhibitor fluridone(FL)and GA_(3)(GA),respectively.Then seeds of different treatments were detected with UPLC-MS/MS.The results showed that the whole treated process of N.incisum seeds could be divided into three stages:0—60 d,90—(150+30)d and(150+60)—(150+110)d,among which N.incisum seeds treated with pretreatment+CS for(150+60)and(150+110)d had germination capablity.The compounds represented by the principal component t[1]were the compounds that had undergone major changes of seed embryos during 0,15,60,90,120 d after pretreatments.The compounds represented by the principal component of t[2]were mainly changed during the release of physiological dormancy.The difference analysis showed that the content of orobanchyl acetate,a strigolactone compound,was accumulated significantly in released dormancy of N.incisum seed,which was increased by 12.1 times after FL treatment and 28.5 times after CS treatment.Therefore,orobanchyl acetate could be crucial during the dormancy release of N.incisum seeds.This study provided a theoretical basis for dormancy release mechanism of N.incisum seeds and a practical significance for the development of Chinese medicine industry of N.incisum.

关 键 词：羌活 种子休眠 代谢组 独脚金内脂 列当醇乙酸酯 

分 类 号：S330.33[农业科学—作物遗传育种]