苍白球大麻素对大鼠运动行为的调控及受体机制  

REGULATORY EFFECT OF INTRAPALLIDAL CANNABINOID ON MOTOR BEHAVIOR OF RATS AND RELATED RECEPTOR MECHANISM

作　　者：于锦锦 薛雁 刘翠 陈蕾 YU Jinjin;XUE Yan;LIU Cui;CHEN Lei(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2021年第2期178-181,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金项目(31671076)。

摘　　要：目的探究大鼠苍白球大麻素对运动行为的影响及受体机制。方法将70只大鼠随机分为10组,单侧或双侧苍白球分别微量注射含二甲基亚砜(DMSO)的人工脑脊液、人工合成大麻素WIN 55,212-2、大麻素1型受体阻断剂AM 251、大麻素2型受体阻断剂AM 630、WIN 55,212-2+AM 251、WIN 55,212-2+AM 630。采用爬杆实验和提升躯体摇摆实验观察大麻素对正常大鼠运动行为的影响。结果双侧苍白球微量注射药物后,WIN 55,212-2组大鼠爬杆时间较对照组明显缩短(F=9.436,t=4.941,P<0.01);与WIN 55,212-2组相比,联合给予AM 251和WIN 55,212-2可明显阻断WIN 55,212-2对正常大鼠爬杆时间的影响(t=3.565,P<0.05),而联合给予AM 630和WIN 55,212-2不能阻断WIN 55,212-2的效应(t=0.514,P>0.05)。单侧苍白球微量注射WIN 55,212-2可引起大鼠明显的对侧摇摆(Z=3.641,P<0.01);联合给予AM 251和WIN 55,212-2可明显阻断WIN 55,212-2诱导的对侧摇摆(Z=3.416,P<0.01),然而这一效应并没有被AM 630阻断(Z=0.764,P>0.05)。结论苍白球给予人工合成大麻素WIN 55,212-2可通过激活大麻素1型受体增强正常大鼠的运动行为。Objective To investigate the effect of intrapallidal cannabinoid on motor behavior of rats and the related receptor mechanism.Methods A total of 70 rats were randomly divided into ten groups,and the globus pallidus at unilateral or bilateral sides was given microinjection of artificial cerebrospinal fluid containing dimethyl sulfoxide,WIN 55,212-2(synthetic cannabinoid),AM 251(cannabinoid receptor type 1 antagonist),AM 630(cannabinoid receptor type 2 antagonist),WIN 55,212-2+AM 251,or WIN 55,212-2+AM 630.The pole test and the elevated body swing test were used to observe the effect of cannabinoid on the motor behavior of normal rats.Results After the microinjection of drugs into the globus pallidus at bilateral sides,the WIN 55,212-2 group had a significant reduction in the time to reach the floor compared with the control group(F=9.436,t=4.941,P<0.01),and compared with the WIN 55,212-2 group,the administration of AM 251 and WIN 55,212-2 significantly blocked the influence of WIN 55,212-2 on the time to reach the floor(t=3.565,P<0.05),while the administration of AM 630 and WIN 55,212-2 did not block the influence of WIN 55,212-2(t=0.514,P>0.05).The microinjection of WIN 55,212-2 into the globus pallidus at unilateral side induced significant contralateral-biased swing in rats(Z=3.641,P<0.01),and the administration of AM 251 and WIN 55,212-2 significantly blocked the contralateral-biased swing induced by WIN 55,212-2(Z=3.416,P<0.01),while such effect was not blocked by AM 630(Z=0.764,P>0.05).Conclusion Administration of the synthetic cannabinoid WIN 55,212-2 into the globus pallidus enhances the motor behavior of normal rats by activating cannabinoid receptor type 1.

关 键 词：苍白球 大麻酚类 运动活动 受体 大麻酚 CB1 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]