伊拉地平对Fe^(2+)诱导MES23.5细胞毒性的作用  

EFFECT OF ISRADIPINE ON FE^(2+)-INDUCED CYTOTOXICITY IN MES23.5 CELLS

作　　者：赵莎 马泽刚[1] ZHAO Sha;MA Zegang(Department of Physiology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学教研室,山东青岛266071

出　　处：《青岛大学学报（医学版）》2021年第2期190-193,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金资助项目(81671249)。

摘　　要：目的探讨L型Ca 2+通道阻断剂伊拉地平对硫酸亚铁诱导的MES23.5细胞毒性的作用。方法以MES23.5多巴胺能细胞系为观察对象,利用流式细胞仪筛选Fe^(2+)最适浓度,伊拉地平则选用实验室前期筛选浓度5μmol/L。应用免疫印迹法(Western blot)检测与凋亡相关蛋白Bcl-2和Bax的表达,初步评估伊拉地平对Fe^(2+)诱导的细胞毒性的保护作用。结果与对照组相比,40μmol/L Fe^(2+)组线粒体膜电位(ΔΨm)明显下降,差异具有统计学意义(F=68.190,q=8.898,P<0.001)。与对照组相比,单独加Fe^(2+)组细胞Bcl-2/Bax蛋白表达比值明显降低(F=6.856,q=6.055,P<0.01),而伊拉地平预处理可改善由Fe^(2+)造成的Bcl-2/Bax蛋白表达比值降低的现象,差异具有统计学意义(q=4.103,P<0.05)。结论伊拉地平预处理可以抑制Bcl-2/Bax蛋白表达比值降低,对Fe^(2+)诱导的细胞损伤可能具有保护作用。Objective To investigate the effect of isradipine,a blocker of L-type Ca 2+channels,on cytotoxicity induced by ferrous sulfate heptahydrate in MES23.5 cells.Methods The MES23.5 dopaminergic cell line was selected for observation.Flow cytometry was used to screen out the optimal concentration of Fe^(2+),and a concentration of 5μmol/L was determined for isradipine in previous laboratory analysis.Western blot was used to measure the expression of the apoptosis-related proteins Bcl-2 and Bax,and the protective effect of isradipine against Fe^(2+)-induced cytotoxicity was evaluated.Results Compared with the control group,the 40μmol/L Fe^(2+)group had a significant reduction in mitochondrial membrane potential(ΔΨm)(F=68.190,q=8.898,P<0.001).Compared with the control group,the Fe^(2+)alone group had a significant reduction in Bcl-2/Bax ratio(F=6.856,q=6.055,P<0.01),while pretreatment with isradipine significantly improved the reduction in Bcl-2/Bax ratio caused by Fe^(2+)(q=4.103,P<0.05).Conclusion Pretreatment with isradipine can inhibit the reduction in Bcl-2/Bax ratio and thus exert a protective effect against cell injury induced by Fe^(2+).

关 键 词：伊拉地平 钙通道阻滞药 铁 帕金森病 神经保护 

分 类 号：R338.2[医药卫生—人体生理学]