枸橼酸铁铵和铁蛋白对原代培养腹侧中脑神经元VMAT-2和DAT表达影响  

EFFECT OF FERRIC AMMONIUM CITRATE AND FERRITIN ON THE EXPRESSION OF VESICULAR MONOAMINE TRANSPORTER 2 AND DOPAMINE TRANSPORTER IN PRIMARY CULTURED VENTRAL MIDBRAIN NEURONS

作　　者：肖志新 宋立梅 谢俊霞 徐华敏 XIAO Zhixin;SONG Limei;XIE Junxia;XU Huamin(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2021年第2期194-197,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金项目(31871202);山东省自然科学基金项目(ZR2019MC057)。

摘　　要：目的探究枸橼酸铁铵(FAC)、含铁铁蛋白(Ferritin)和去铁铁蛋白(Apoferritin)对原代培养的腹侧中脑神经元中单胺囊泡转运蛋白-2(VMAT-2)和多巴胺转运蛋白(DAT)表达的影响。方法以FAC、Ferritin和Apoferritin处理原代培养的腹侧中脑神经元24 h后,应用蛋白质免疫印迹(Western Blot)方法检测神经元中VMAT-2和DAT的表达情况。结果FAC、Ferritin和Apoferritin处理的原代腹侧中脑神经元VMAT-2表达较对照组明显降低,差异具有统计学意义(F=4.295,P<0.05),而DAT的表达没有明显变化。结论FAC、Ferritin和Apoferritin能够降低原代培养的腹侧中脑神经元的VMAT-2表达,而对DAT表达没有明显影响。Objective To investigate the effect of ferric ammonium citrate(FAC),Ferritin,and Apoferritin on the expression of vesicular monoamine transporter 2(VMAT-2)and dopamine transporter(DAT)in primary cultured ventral midbrain neurons.Methods After primary cultured ventral midbrain neurons were treated with FAC,Ferritin,or Apoferritin for 24 h,Western blot was used to measure the expression of VMAT-2 and DAT in neurons.Results Compared with the control group,the group of primary cultured ventral midbrain neurons treated by FAC,Ferritin,or Apoferritin had a significant reduction in the expression of VMAT-2(F=4.295,P<0.05),while there was no significant change in the expression of DAT.Conclusion FAC,Ferritin,and Apoferritin can reduce the expression of VMAT-2 in primary cultured ventral midbrain neurons,with no significant effect on the expression of DAT.

关 键 词：铁 铁蛋白质类 中脑 神经元 囊泡单胺转运蛋白质类 多巴胺质膜转运蛋白质类 

分 类 号：R338.2[医药卫生—人体生理学]