MPTP诱导PD小鼠黑质及前额叶皮质铁相关蛋白表达变化  

CHANGES IN THE EXPRESSION OF IRON-RELATED PROTEINS IN THE SUBSTANTIAL NIGRA AND THE PREFRONTAL CORTEX OF MICE WITH 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE-INDUCED PARKINSON DISEASE

作　　者：宋立梅 肖志新 谢俊霞 徐华敏 SONG Limei;XIAO Zhixin;XIE Junxia;XU Huamin(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2021年第2期198-201,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金项目(31871202);山东省自然科学基金项目(ZR2019MC057)。

摘　　要：目的研究1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)小鼠模型黑质(SN)及内侧前额叶皮质(mPFC)铁代谢相关蛋白的表达变化。方法将12只9周龄C57BL/6J雄性小鼠随机分为对照组和MPTP组。对照组小鼠给予生理盐水腹腔注射,MPTP组小鼠给予MPTP 30 mg/kg腹腔注射,5 d后进行爬杆实验检测小鼠的运动协调能力,Western Blot检测铁代谢相关蛋白的表达变化。结果爬杆实验结果显示,与对照组相比,MPTP组小鼠转头时间和下杆时间均明显增加,差异有统计学意义(t=2.420、3.464,P<0.05)。Western Blot结果显示,与对照组相比,MPTP组小鼠SN区的重链铁蛋白(H-Ferritin)和二价金属离子转运蛋白1(DMT1)表达明显增加(t=3.969、3.333,P<0.01),转铁蛋白受体1(TfR1)表达明显下降(t=2.318,P<0.05);两组mPFC区的DMT1、TfR1和H-Ferritin表达差异均无显著性。结论MPTP能够改变小鼠SN区的铁代谢相关蛋白的表达,但并不影响mPFC区铁代谢相关蛋白的表达。Objective To investigate the changes in the expression of iron metabolism-related proteins in the substantia nigra(SN)and the medial prefrontal cortex(mPFC)of mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson disease(PD).Methods A total of 12 male C57BL/6J mice,aged 9 weeks,were randomly divided into control group and MPTP group.The mice in the control group were given intraperitoneal injection of normal saline,and those in the MPTP group were given intraperitoneal injection of MPTP 30 mg/kg.After 5 days,the pole test was used to observe motor coordination ability,and Western blot was used to measure the expression of iron metabolism-related proteins.Results The results of the pole test showed that compared with the control group,the MPTP group had significant increases in the time to turn around and the time to climb down the pole(t=2.420,3.464;P<0.05).The results of Western blot showed that compared with the control group,the MPTP group had significant increases in the expression of heavy-chain ferritin and divalent metal transporter 1(DMT1)(t=3.969,3.333;P<0.01)and a significant reduction in the expression of transferrin receptor 1(TfR1)(t=2.318,P<0.05)in the SN of mice,while there were no significant differences between the two groups in the expression of DMT1,TfR1,and heavy-chain ferritin in the mPFC of mice.Conclusion MPTP can change the expression of iron metabolism-related proteins in the SN of mice,but it does not affect the expression of iron metabolism-related proteins in the mPFC of mice.

关 键 词：帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 铁蛋白质类 受体 转铁蛋白 额叶前皮质 黑质 小鼠 

分 类 号：R338.2[医药卫生—人体生理学]