7,8-DHF对大鼠心肌缺血-再灌注损伤影响及机制  

EFFECT OF 7,8-DIHYDROXYFLAVONE ON MYOCARDIAL ISCHEMIA-REPERFUSION INJURY IN RATS AND ITS MECHANISM

作　　者：李浩然 迟晓琦 吴雪[1] 张文秀 韩晓华[1] LI Haoran;CHI Xiaoqi;WU Xue;ZHANG Wenxiu;HAN Xiaohua(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2021年第2期210-213,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省自然科学基金项目(ZR2019MH110);青岛市民生科技计划项目(19-6-1-19-nsh)。

摘　　要：目的探讨7,8-二羟基黄酮(7,8-DHF)对大鼠心肌缺血-再灌注(I/R)损伤的影响及机制。方法选取雄性健康Wistar大鼠18只,随机分为3组,每组6只。假手术组(Sham组)大鼠开胸,左冠状动脉前降支(LAD)只穿线不结扎;I/R组先结扎LAD缺血30 min,再恢复血供3 h;I/R+7,8-DHF组先缺血30 min,恢复血供前10 min腹腔注射7,8-DHF(10 mg/kg)。心脏恢复血供3 h后处死大鼠,对血清中乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)的活力进行检测,取结扎线下方的心肌组织采用Western blot方法检测与凋亡有关的蛋白。结果与Sham组相比较,I/R组大鼠血清中LDH和CK-MB的活力分别升高35%和71%,7,8-DHF预处理则部分抑制了上述变化(F=18.770、5.422,q=3.788~7.800,P<0.05)。Western blot检测显示,与Sham组相比,I/R组大鼠心肌组织中Bcl-2蛋白表达显著降低,而Bax和cleaved caspase-3蛋白表达显著增加;与I/R组相比,I/R+7,8-DHF组Bcl-2蛋白表达显著升高,Bax和cleaved caspase-3蛋白表达显著减少(F=6.217~14.720,q=3.797~7.546,P<0.05)。结论7,8-DHF预处理对大鼠的心肌I/R损伤具有保护作用,该作用可能与其抑制心肌细胞凋亡有关。Objective To investigate the effect of 7,8-dihydroxyflavone(7,8-DHF)on myocardial ischemia-reperfusion(I/R)injury in rats and its mechanism.Methods A total of 18 healthy male Wistar rats were selected and randomly divided into sham-operation group(sham group),I/R group,and I/R+7,8-DHF group,with 6 rats in each group.The rats in the sham-operation group were given thoracotomy without ligation of the left anterior descending coronary artery(LAD),those in the I/R group were given ligation of the LAD for 30 min of ischemia,followed by the restoration of blood supply for 3 h,and those in the I/R+7,8-DHF group were given ischemia for 30 min,followed by intraperitoneal injection of 7,8-DHF(10 mg/kg)at 10 min before the restoration of blood supply.The rats were sacrificed at 3 h after the restoration of blood supply,and the serum levels of lactate dehydrogenase(LDH)and creatine kinase-MB(CK-MB)were measured.The myocardial tissue below the ligature was collected to measure apoptosis-related proteins by Western blot.Results Compared with the sham group,the I/R group had significant increases in the serum levels of LDH and CK-MB(increased by 35%and 71%,respectively),and these changes were partially inhibited by 7,8-DHF pretreatment(F=18.770 and 5.422,q=3.788-7.800,P<0.05).Western blot showed that compared with the sham group,the I/R group had a significant reduction in the protein expression of Bcl-2 and significant increases in the protein expression of Bax and cleaved caspase-3 in myocardial tissue;compared with the I/R group,the I/R+7,8-DHF group had a significant increase in the protein expression of Bcl-2 and significant reductions in the protein expression of Bax and cleaved caspase-3(F=6.217-14.720,q=3.797-7.546,P<0.05).Conclusion Pretreatment with 7,8-DHF has a protective effect against myocardial I/R injury in rats,possibly by inhibiting cardiomyocyte apoptosis.

关 键 词：黄酮类 心肌再灌注损伤 细胞凋亡 大鼠 

分 类 号：R337.1[医药卫生—人体生理学] R542.2[医药卫生—基础医学]