疏肝解郁法联合针刺对糖尿病合并抑郁大鼠血糖、胰岛素受体及ERK1/2、CaMK信号通路表达的影响  

Effect of Shugan Jieyu Fang combined with acupuncture on blood glucose,insulin receptor,ERK1/2 and CaMK signaling pathway expression in diabetic rats with depression

作　　者：房丹 FANG Dan(Fourth Department of Internal Medicine,Heping Hospital of the 983rd Hospital of the Joint Logistics Support Forces,Tianjin 300020,China)

机构地区：[1]联勤保障部队第九八三医院和平院区内四科,天津300020

出　　处：《吉林中医药》2021年第4期505-509,共5页Jilin Journal of Chinese Medicine

基　　金：天津市科技发展计划项目(2019A03058)。

摘　　要：目的探讨疏肝解郁法联合针刺对糖尿病合并抑郁大鼠血糖、胰岛素受体及ERK1/2、CaMK信号通路表达的影响。方法选择SPF级雄性大鼠48只,随机选取8只作为空白组(A组),给予标准饲料饲养;其余40只则为造模组,给予高糖高脂饲料饲养。造模组所有大鼠通过腹腔内注射STZ溶液、慢性孤养和不可预见性中等刺激造模,建立糖尿病合并抑郁大鼠模型,并将其随机分为5组,分别为模型组(B组)、阳性对照组(C组)、针刺组(D组)、疏肝解郁方组(E组)和疏肝解郁方联合针刺组(F组)。A组与B组给予等剂量生理盐水灌胃;C组给予临床等效剂量二甲双胍(0.18 g/kg)加百忧解(1.8 mg/kg)灌胃治疗;D组统一于每天早上10点进针刺百会、后三里、三阴交;E组给予疏肝解郁方(柴胡6 g,陈皮6 g,川芎6 g,党参8 g,白术8 g,茯苓8 g,香附5 g,白芍5 g,甘草3 g)临床等效剂量3.5 g/kg标准灌胃;F组治疗方法为针刺加疏肝解郁方。治疗7 d后测定空腹血糖(FBG)、糖化血红蛋白(HbA1c)、胰岛素抵抗指数(HOMA-IR),RT-PCR法检测胰岛素受体(IR)、胰岛素底物-1(IRS-1)mRNA表达,Western-bloting法检测大鼠海马组织CaM、CaMKⅡ、ERK1/2表达。结果与A组比较,B组FBG、HbA1c、HOMA-IR均明显升高(P<0.05),肝组织IR、IRS-1 mRNA和海马组织CaM、CaMKⅡ、ERK1/2明显降低(P<0.05);与B组比较,C、D、E、F组FBG、HbA1c、HOMA-IR、IR、IRS-1 mRNA、CaM、CaMKⅡ、ERK1/2明显统计学差异(P<0.05);F组FBG、HbA1c、HOMA-IR、IR、IRS-1 mRNA、CaM、CaMKⅡ、ERK1/2与D、E组比较有显著差异(P<0.05),除CaMKⅡ表达外与C组比较无明显差异(P>0.05)。结论疏肝解郁法联合针刺治疗糖尿病合并抑郁大鼠的机制可能与改善血糖异常,提高肝组织IR、IRS-1的基因表达从而改善胰岛素的信号传导,激活ERK1/2、CaMK信号通路调节大鼠海马神经元再生有关。Objective To explore the effect of Shugan Jieyu Fang combined with acupuncture on blood glucose,insulin receptor,ERK1/2 and CaMK signaling pathway expression in diabetic rats with depression.Methods A total of 48 SPF male rats were used as subjects in the study,with 8 of them randomly selected as the blank group(group A)and fed with standard diet.The remaining 40 rats in the model group were fed with high sugar and high fat diet.The model of diabetic rats with depression was made by cavity injection of STZ solution,chronic solitary raising and unpredictable medium-degree stimulation.The modeled rats were further randomly divided into five groups,respectively model group(group B),positive control group(group C),acupuncture group(group D),Shugan Jieyu Fang group(group E)and Shugan Jieyu Fang combined with acupuncture group(Group F).Group A and group B were given the same dose of normal saline by gavage.Group C was given the clinical equivalent dose of metformin(0.18 g/kg)plus prozac(1.8 mg/kg)by gavage.Group D was given acupuncture treatment of Baihui,Housanli and Sanyinjiao at 10 a.m.every day.Group E was treated with Shugan Jieyu Fang(Chinese thorowax root 6 g,dried tangerine peel 6 g,Sichuan lovage rhizome 6 g,tangshen 8 g,largehead atractylodes rhizome 8 g,Indian bread 8 g,nutgrass galingale rhizome 5 g,debark peony root 5 g,liquorice root 3 g)with the clinical equivalent dose of 3.5 g/kg by gavage,and the treatment of group F was acupuncture plus Shugan Jieyu Fang.After 7 days of treatment,fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c)and insulin resistance index(HOMA-IR)were measured.The mRNA expressions of insulin receptor(IR)and insulin substrate-1(IRS-1)were detected by RT-PCR.The expressions of CaM,CaMKⅡand ERK1/2 in rat hippocampus were detected by Western-blotting.Results Compared with group A,FBG,HbA1c and HOMA-IR in group B were significantly increased(P<0.05),while IR,IRS-1 mRNA in the liver tissues and CaM,CaMKⅡand ERK1/2 in the hippocampus tissues were significantly decreased(P<0.05)

关 键 词：疏肝解郁法 针刺 糖尿病合并抑郁 大鼠 血糖 胰岛素受体 ERK1/2 CAMK 

分 类 号：R285.5[医药卫生—中药学]