天门冬离体快繁技术  

Rapid Propagation in vitro of Asparagus cochinchinensis

作　　者：李林 李扬 张明生[1] 彭思静 吴尚浇 贾娜 刘智 于晓松 杨朝雄 LI Lin;LI Yang;ZHANG Ming-sheng;PENG Si-jing;WU Shang-jiao;JIA Na;LIU Zhi;YU Xiao-song;YANG Chao-xiong(School of Life Sciences/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region,Ministry of Education,Guizhou University,Guiyang 550025,Guizhou China;Guizhou Xiancao Agricultural Group Limited Company,Guiyang 550081,Guizhou China)

机构地区：[1]贵州大学生命科学学院/山地植物资源保护与种质创新教育部重点实验室,贵州贵阳550025 [2]贵州仙草农业集团有限公司,贵州贵阳550081

出　　处：《亚热带植物科学》2021年第1期46-50,共5页Subtropical Plant Science

基　　金：国家重点研发计划课题(2016YFC0502604);贵州省科技计划重大专项课题(黔科合平台人才[2017]5411-06);国家喀斯特石漠化防治工程技术研究中心建设项目(2012FU125X13);贵州省高层次创新型人才培养计划项目(黔科合人才[2015]4031号);贵州省中药材现代产业技术体系建设项目(GZCYTX-02)。

摘　　要：以无菌萌发的天门冬(Asparagus cochinchinensis)种子胚轴为外植体,研究植物生长调节物质种类及浓度对愈伤组织诱导、丛生芽分化和植株再生的影响,建立其离体快速繁殖技术。结果表明,愈伤组织诱导的适宜培养基为MS+6-BA 1.0 mg·L^(-1)+NAA 0.5 mg·L^(-1),诱导率95.6%;愈伤组织增殖培养基为MS+6-BA 0.5 mg·L^(-1)+NAA 2.0 mg·L^(-1),增殖倍数为9.7;丛生芽诱导培养基为MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+KT 0.1 mg·L^(-1),诱导率为91.1%;适宜的壮苗培养基为MS+6-BA 0.2 mg·L^(-1)+IAA 1.0 mg·L^(-1);适宜的生根培养基为1/2MS+NAA 2.0 mg·L^(-1),生根率达84.4%。本研究为构建天门冬药材产业化所需种苗生产技术体系奠定了基础。In order to establish the rapid propagation technique in vitro of Asparagus cochinchinensis,the hypocotyls in seeds by aseptic sprouting were used as explants,the effects of different plant growth regulators on callus induction,shoots differentiation and plants regeneration were studied.The results showed that the suitable medium for callus induction was MS+6-BA 1.0 mg·L^(-1)+NAA 0.5 mg·L^(-1),and the induction rate was 95.6%;The proliferation medium of callus was MS+6-BA 0.5 mg·L^(-1)+NAA 2.0 mg·L^(-1),and the proliferation multiple was 9.7;The induction medium of clustered buds was MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1)+KT 0.1 mg·L^(-1),the induction rate was 91.1%;The suitable medium for strong seedlings was MS+6-BA 0.2 mg·L^(-1)+IAA 1.0 mg·L^(-1);The suitable rooting medium was 1/2 MS+NAA 2.0 mg·L^(-1),the rooting rate reached 84.4%.This study laid a foundation for the technical system construction of seedlings production in the industrialization of A.cochinchinensis.

关 键 词：天门冬 组织培养 愈伤组织 丛生芽 快速繁殖 

分 类 号：S682.36[农业科学—观赏园艺]