野生云南山楂果实化学成分研究  

Chemical Constituents of the Fruits of Wild Crataegus scabrifolia

作　　者：朱起杰 郎利娟 姜北 沈怡 王影 肖朝江 Zhu Qijie;Lang Lijuan;Jiang Bei;Shen Yi;Wang Ying;Xiao Chaojiang(Institute of Materia Medica,Dali University,Dali,Yunnan 671000,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药物研究所,云南大理671000 [2]大理大学药学院,云南大理671000

出　　处：《大理大学学报》2021年第4期7-9,共3页Journal of Dali University

基　　金：云南省地方本科高校(部分)基础研究联合专项资金项目(2019FH001-050);大理大学滇西特色药用植物与白族药开发利用创新团队项目(ZKLX2019106);大理大学博士科研启动费项目(KYBS201734)。

摘　　要：目的:研究野生云南山楂果实中的化学成分及其降脂活性。方法:运用硅胶、Sephadex LH-20等柱色谱对化合物进行分离纯化,并通过理化性质及现代波谱分析等手段对化合物进行结构鉴定;采用高脂HepG2细胞模型进行体外降脂活性测试。结果:从野生云南山楂果实95%乙醇提取物中分离鉴定了6个单体化合物,分别为苯甲酸(1)、2-羟基丁二酸二甲酯(2)、2-羟基丁二酸-1-甲基酯(3)、β-谷甾醇(4)、胡萝卜苷(5)、槲皮素(6);化合物2~5在200μmol/L浓度下均未显示出明显的降脂率。结论:化合物2和3为首次从山楂属植物中分离得到,化合物1为首次从该植物中分离得到,所有供试化合物均无体外降脂活性。Objective:To study the chemical constituents of Crataegus scabrifolia(Franch.)Rehd.and their lipid-lowering activities.Methods:The compounds were isolated and purified by column chromatography padded with silica gel and Sephadex LH-20,and their structures were identified based on the comprehensive analyses of the physicochemical properties and spectral data.The lipid-lowering activity in vitro was evaluated by cellular model of high-fat HepG2.Results:Six compounds were obtained from the 95%ethanol extract of the fruits of wild C.scabrifolia and identified as benzoic acid(1),2-hydroxybutanedioic acid dimethyl ester(2),2-hydroxy-succinic acid 1-methyl ester(3),β-sitosterol(4),daucosterol(5),and quercetin(6).Compounds 2-5 showed no significant lipid reduction at 200μmol/L concentration.Conclusion:This was the first report on compounds 2 and 3 from the plants of Genus Crataegus,and compound 1 was isolated from C.scabrifolia for the first time.Compounds 2-5 had no lipid-lowering activity in vitro.

关 键 词：云南山楂 化学成分 2-羟基丁二酸 

分 类 号：R284.1[医药卫生—中药学]