美洲大蠊活性提取物经胃、肠道给药后的抗肝纤维化作用比较  

Effects of Active Extracts from Periplaneta americana on Treating Hepatic Fibrosis in Rats by Gastric and Intestinal Administration

作　　者：顾婷 杨永寿 谢静静 肖培云 Gu Ting;Yang Yongshou;Xie Jingjing;Xiao Peiyun(College of Pharmacy,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药学院,云南大理671000

出　　处：《大理大学学报》2021年第4期18-22,共5页Journal of Dali University

基　　金：国家自然科学基金项目(82060747;81560634);云南省地方本科高校(部分)基础研究联合专项资金项目(2019FH001-034)。

摘　　要：目的:研究美洲大蠊活性提取物(PA-B)经胃、肠给药后对四氯化碳所致肝纤维化大鼠模型的影响。方法:雄性SD大鼠按体质量随机分为对照组、模型组、肠溶辅料组、胃溶辅料组、阳性药物组、原料药组、肠溶药物组、胃溶药物组,除对照组外其余各组腹腔注射40%四氯化碳油剂,对照组注射同等剂量橄榄油。造模成功后,各组给予相应药物。对肝组织进行苏木精-伊红(HE)染色、Masson染色,观察病理学改变。微板法测定丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST),酶联免疫吸附(ELISA)法测定透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PC-Ⅲ)、Ⅳ型胶原蛋白(Col-Ⅳ),碱水解法测定羟脯氨酸(HYP)的含量。结果:与对照组比较,模型组胶原纤维增多,假小叶明显;与模型组比较,阳性药物组、原料药组、肠溶药物组、胃溶药物组各项检测指标水平降低,差异均有统计学意义(P<0.05或P<0.01);阳性药物组、原料药组、肠溶药物组、胃溶药物组间各指标差异均无统计学意义(P>0.05)。结论:PA-B及相关制剂均能有效改善大鼠肝纤维化,但药物经胃、肠不同途径吸收,对肝纤维化大鼠的治疗作用无显著差异。Objective:To study the effects of active extract of Periplaneta americana(PA-B)on liver fibrosis induced by carbon tetrachloride in rats by gastric and intestinal administration.Methods:According to the body weight,male SD rats were randomly divided into 8 groups,including the control group,the model group,enteric-soluble excipient group,gastric-soluble excipient group,the positive drug group,the active pharmaceutical ingredients(APIs)group,enteric drug group and gastric drug group.Except for the control group,the other groups were intraperitoneally injected with 40%carbon tetrachloride oil,the control group was injected with the same dose of olive oil.After successful modeling,each group was given corresponding drugs.The liver tissues were stained by hematoxylin-eosin(HE)and Masson,and the pathological changes were observed under the microscope.The microplate method was used to determine alanine aminotransferase(ALT)and aspartate aminotransferase(AST);the enzyme-linked immunosorbent assay(ELISA)was applied to measure serum liver fibrosis indexes hyaluronic acid(HA),laminin(LN),procollagen typeⅢ(PC-Ⅲ)and collagen typeⅣ(Col-Ⅳ);the content of hydroxyproline(HYP)in liver tissue was measured by the alkaline hydrolysis method.Results:Compared with the control group,the model group had more collagen fibers and more false lobules;compared with the model group,the levels of various detection indexes in the positive drug group,the APIs group,enteric drug group and gastric drug group decreased,and the differences were statistically significant(P<0.05 or P<0.01).There was no statistical significance in each index among positive drug group,APIs group,enteric drug group and gastric drug group(P>0.05).Conclusion:PA-B and related preparations could effectively improve liver fibrosis in rats,but there was no significant difference in the therapeutic effect of PA-B on liver fibrosis in rats by gastric and intestinal administration.

关 键 词：美洲大蠊 肝纤维化 四氯化碳 给药途径 

分 类 号：R963[医药卫生—微生物与生化药学] R-332[医药卫生—药理学]