基于网络药理学分析牡丹皮-赤芍治疗银屑病的作用机制  

Study on the mechanism of cortex moutan-radix paeoniae rubra in the treatment of psoriasis based on network pharmacology

作　　者：陈广山[1] 李玲玲[1] 白春晓[1] CHEN Guangshan;LI Lingling;BAI Chunxiao(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《天津中医药》2021年第5期659-665,共7页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81874393)。

摘　　要：[目的]通过网络药理学方法分析牡丹皮-赤芍治疗银屑病的有效成分、作用靶点及作用通路,探讨其作用机制。[方法]通过中药系统药理数据库和分析平台(TCMSP),检索牡丹皮和赤芍的有效成分以及相应的作用靶点,通过The Human Gene Database(GeneCards)平台检索银屑病的相关靶点,利用ClusterProfiler R软件绘制韦恩图,获得牡丹皮-赤芍与银屑病的共同靶点。利用STRING平台和Cytoscape3.7.2软件,构建靶蛋白相互作用网络和药物-有效成分-靶点-疾病网络。利用ClusterProfiler R软件平台对牡丹皮-赤芍及银屑病的共同作用靶点进行GO功能富集分析和KEGG代谢通路富集分析。[结果]筛选出牡丹皮-赤芍治疗银屑病的有效成分37个和潜在作用靶点112个,关键靶点为JUN、AKT1、RELA,发现细胞因子受体结合、细胞因子活性、血红素结合等生物学过程和AGE-RAGE信号通路、白介素(IL)-17信号通路、肿瘤坏死因子(TNF)信号通路等在牡丹皮-赤芍治疗银屑病中起到较为关键的作用。[结论]牡丹皮-赤芍治疗银屑病是通过多种有效成分作用在多种靶点、多种通路的生物学过程实现的,也为进一步研究牡丹皮-赤芍治疗银屑病的作用机制提供了理论基础。[Objective]To analyze the effective components,targets and pathways of cortex moutan-radix paeoniae rubra in the treatment of psoriasis by network pharmacology,and to explore its mechanism of action.[Methods]The effective components and corresponding action targets of cortex moutan and radix paeoniae rubra were searched through TCMSP platform,the related targets of psoriasis were searched through GeneCards platform.Wayne diagram was drawn by ClusterProfiler R software,so as to obtain the common targets of cortex moutan-radix paeoniae rubra and psoriasis.Using STRING platform and Cytoscape 3.7.2 software,the target protein interaction network and drug-active ingredient-target-disease network were constructed.GO function enrichment analysis and KEGG metabolic pathway enrichment analysis were carried out on the common targets of cortex moutan-radix paeoniae rubra and psoriasis by using ClusterProfiler R software platform.[Results]Thirty-seven effective components and 112 potential targets were screened out,among which JUN,AKT1 and RELA were the most concentrated targets.It was found that biological processes such as cytokine receptor binding,cytokine activity and heme binding,and AGE-RAGE signal pathway,IL-17 signal pathway and TNF signal pathway played a key role in the treatment of psoriasis with cortex moutan-radix paeoniae rubra.[Conclusion]The treatment of psoriasis by cortex moutan-radix paeoniae rubra is realized by the biological process of multiple effective components acting on multiple targets and pathways,which also provides a theoretical basis for further study on the mechanism of cortex moutan-radix paeoniae rubra in treating psoriasis.

关 键 词：牡丹皮 赤芍:银屑病 网络药理学 靶点 作用通路 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]