根瘤菌与固氮菌联合对毛叶苕子的促生效果  

Co-inoculation with rhizobia and azotobacter affects the growth of Vicia villosa

作　　者：魏志敏 孙斌 方成 代子雯 刘满强[1] 焦加国[1] 胡锋[1] 李辉信[1] 徐莉[1] WEI Zhi-min;SUN Bin;FANG Cheng;DAI Zi-wen;LIU Man-qiang;JIAO Jia-guo;HU Feng;LI Hui-xin;XU Li(College of Resources and Environment Sciences,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学资源与环境科学学院,江苏南京210095

出　　处：《草业学报》2021年第5期94-102,共9页Acta Prataculturae Sinica

基　　金：国家绿肥产业技术体系项目(CARS-22-G-10);公益性行业(农业)科研专项经费(201503121);江苏省优势学科项目资助。

摘　　要：利用前期筛选的具有优良固氮活性的巨大芽孢杆菌菌株N3和豌豆根瘤菌菌株Vic5,研究单一及复合接种对毛叶苕子的促生效果及对土壤性质的影响。结果表明:相比不接菌处理CK,单一接种N3能显著提高毛叶苕子根瘤固氮酶活性,单一接种Vic5能显著提高毛叶苕子地上部生物量、结瘤数和根瘤固氮酶活性,Vic5处理下毛叶苕子地上部生物量、结瘤数、根瘤固氮酶活性及根系形态学指标包括根系总长、根表面积、根体积及根尖数较N3处理分别增加了72.55%、121.20%、47.22%、21.46%、50.48%、53.85%和47.42%。相比CK,接种N3处理土壤全磷、速效磷和速效钾含量显著提高,Vic5处理土壤铵态氮和硝态氮含量显著提高,复合接菌处理下土壤有机质和全氮含量显著提高。接种N3能显著提高土壤固氮酶活性,接种Vic5能显著提高土壤基础呼吸、微生物量碳、氮含量和土壤固氮酶活性,复合接种处理下土壤基础呼吸强度最大、微生物量碳、氮含量最高,土壤固氮酶活性最高,用乙炔还原法测得固氮酶活性为C_2H_453.77 nmol·g^(-1)·h^(-1)。综上所述,相比单一接种,复合接种自生固氮菌N3和豌豆根瘤菌Vic5表现出对毛叶苕子更优的促生作用和对土壤具有更好的改良作用,可为后期毛叶苕子专性生物菌肥的开发利用奠定基础。The effects of single or combined inoculation of the nitrogen-fixing bacteria Bacillus megaterium N3(hereafter N3)and Rhizobium leguminosarum Vic5(hereafter Vic5)on the growth of Vicia villosa and on soil properties were evaluated in this study.The results showed that,compared with the uninoculated control,V.villosa plants inoculated with strain N3 showed significantly increased nodule nitrogenase activity,and those inoculated with strain Vic5 showed significantly increased aboveground biomass,nodule numbers,and the nodule nitrogenase activity.The aboveground biomass,nodule numbers,nodule nitrogenase activity,and root morphological indexes including root length,root surface area,root volume,and root number of V.villosa were significantly higher(72.55%,121.20%,47.22%,21.46%,50.48%,53.85%,and 47.42%higher,respectively)in the single Vic5 inoculation treatment than in the single N3 inoculation treatment.Compared with the soil in the control,that in the single N3 inoculation treatment showed significantly higher total phosphorus,available phosphorus,and available potassium contents,and that in the single Vic5 inoculation treatment showed significantly increased ammonium nitrogen and nitrate nitrogen contents.Combined inoculation with both strains significantly increased the contents of soil organic matter and total nitrogen.Single inoculation with strain N3 significantly increased the soil nitrogenase activity,while single inoculation with strain Vic5 significantly increased soil basal respiration,microbial biomass carbon and nitrogen contents,and soil nitrogenase activity.The highest values for soil basal respiration intensity,microbial biomass carbon and nitrogen contents,and soil nitrogenase activity were in the combined inoculation treatment.The soil nitrogenase activity measured by acetylene reduction reached C2H453.77 nmol·g^(-1)·h^(-1).In conclusion,compared with single inoculation,combined inoculation with nitrogen-fixing bacterial strains N3 and Vic5 can promote the growth of V.villosa and improve soil qual

关 键 词：固氮菌 根瘤菌 毛叶苕子 生物菌肥 

分 类 号：S551.2[农业科学—作物学]