利多卡因上调MDC1-AS对膀胱癌细胞T24凋亡、迁移侵袭的影响  

Effect of Lidocaine on Apoptosis,Migration and Invasion of Bladder Cancer Cell T24 by Up-Regulating MDC1-AS

作　　者：丁志刚 DING Zhigang(Dept.of Anesthesiology,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China)

机构地区：[1]首都医科大学附属北京世纪坛医院麻醉科,北京100038

出　　处：《中国医院用药评价与分析》2021年第4期395-400,共6页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：北京市卫生科技发展专项基金(No.2017-1-177)。

摘　　要：目的:探讨利多卡因对膀胱癌细胞T24凋亡、迁移侵袭的影响及其机制。方法:分别通过流式细胞术、Transwell实验分析利多卡因(1.25、2.5及5 mg/ml)对T24细胞凋亡、迁移和侵袭的影响,以正常培养的T24细胞作为对照。实时定量聚合酶链反应分析DNA损伤检测点介质1反义RNA(MDC1-AS)表达量。分析过表达MDC1-AS对T24细胞凋亡、迁移和侵袭的影响,转染MDC1-AS过表达质粒(pcDNA-MDC1-AS)及其对照(pcDNA)、MDC1-AS小干扰RNA(si-MDC1-AS)及其对照(si-NC)至T24细胞,用含5 mg/ml利多卡因的培养液孵育转染si-NC、si-MDC1-AS的T24细胞2 h,分别记为利多卡因5 mg/ml+si-NC组、利多卡因5 mg/ml+si-MDC1-AS组。结果:与对照组比较,2.5、5 mg/ml的利多卡因干预后T24细胞凋亡率、Bax蛋白表达显著升高,Bcl-2蛋白表达量显著降低,迁移和侵袭细胞数显著减少,MDC1-AS表达量显著升高,上述差异均有统计学意义(P<0.05)。与pcDNA组比较,pcDNA-MDC1-AS组T24细胞中MDC1-AS表达量显著升高,细胞凋亡率、Bax蛋白表达量显著升高,迁移和侵袭细胞数、Bcl-2蛋白表达量显著降低,差异均有统计学意义(P<0.05)。与利多卡因5 mg/ml+si-NC组比较,利多卡因5 mg/ml+si-MDC1-AS组T24细胞中MDC1-AS表达量显著降低,细胞凋亡率、Bax蛋白表达量显著降低,迁移和侵袭细胞数、Bcl-2蛋白表达量显著升高,差异均有统计学意义(P<0.05)。结论:利多卡因通过上调MDC1-AS表达从而抑制膀胱癌细胞T24迁移和侵袭,促进细胞凋亡。OBJECTIVE:To probe into the effect of lidocaine on apoptosis,migration and invasion of bladder cancer cell T24 by up-regulating MDC1-AS and its mechanism.METHODS:Flow cytometry and Transwell experiment were used to analyze the effect of lidocaine(1.25,2.5 and 5 mg/ml)on apoptosis,migration and invasion of T24 cells.Normal cultured T24 cells were took as the control group.Real-Time quantitative polymerase chain reaction was used to analyze the expression level of mediator of DNA damage checkpoint protein 1 antisense RNA(MDC1-AS).The effects of over-expression of MDC1-AS on apoptosis,migration and invasion of T24 cells were analyzed.MDC1-AS over-expression plasmid(pcDNA-MDC1-AS)and its control(pcDNA),MDC1-AS small interfering RNA(si-MDC1-AS)and its control(si-NC)were transfected into T24 cells;T24 cells transfected with si-NC and si-MDC1-AS were incubated with the culture medium containing 5 mg/ml lidocaine for 2 h,which were recorded as lidocaine 5 mg/ml+si-NC group and lidocaine 5 mg/ml+si-MDC1-AS group.RESULTS:Compared with the control group,the apoptosis rate of T24 cells and the expression of Bax protein increased significantly,the expression of Bcl-2 protein decreased significantly,the number of migration and invasion cells decreased significantly,and the expression of MDC1-AS increased significantly after treatment with 2.5 and 5 mg/ml lidocaine,with statistically significant difference(P<0.05).Compared with the pcDNA group,the expression of MDC1-AS in T24 cells of the pcDNA-MDC1-AS group increased significantly,the apoptosis rate and the expression of Bax protein increased significantly,the number of migration and invasion cells and the expression of Bcl-2 protein decreased significantly,the differences were statistically significant(P<0.05).Compared with the lidocaine 5 mg/ml+si-NC group,the expression of MDC1-As in T24 cells decreased significantly in the lidocaine 5 mg/ml+si-MDC1-AS group,the apoptosis rate and the expression of Bax protein decreased significantly,while the number of migration and invasi

关 键 词：利多卡因 MDC1-AS 膀胱癌 细胞凋亡 迁移 侵袭 

分 类 号：R979.1[医药卫生—药品] R96[医药卫生—药学]