荧光假单胞菌YG-1与杀菌剂复配对番茄灰霉病的联合毒力  

Combined Virulence of Pseudomonas fluorescens YG-1 and Fungicide Compound on Tomato Gray Mold

作　　者：张萌 翟乾行 朱承余 郑丽宁 张浩 ZHANG Meng;ZHAI Qianhang;ZHU Chengyu;ZHENG Lining;ZHANG Hao(College of Plant Protection,Jilin Agricultural University,Changchun 130118,Jilin,China)

机构地区：[1]吉林农业大学植物保护学院,吉林长春130118

出　　处：《中国蔬菜》2021年第5期70-74,共5页China Vegetables

基　　金：国家重点研发计划支持项目(2019YFD1002000-20)。

摘　　要：采用室内毒力测定方法,明确了8种化学杀菌剂和荧光假单胞菌YG-1对番茄灰霉病菌的毒力作用,并利用平板计数法测定8种杀菌剂与YG-1菌株的生物相容性,筛选生防菌株与杀菌剂复配的最佳配比,通过盆栽试验进一步验证荧光假单胞菌株YG-1与杀菌剂复配对番茄灰霉病的防治效果。研究结果表明,8种杀菌剂中啶酰菌胺和咯菌腈对番茄灰霉病菌的毒力作用较强,EC50值分别为0.081、0.075 mg·L-1,其中啶酰菌胺与荧光假单胞菌YG-1菌株有较好的生物相容性。1.97×108 CFU·mL-1荧光假单胞菌YG-1菌悬液与0.081 mg·L-1啶酰菌胺复配最佳配比为体积比5∶5,对番茄灰霉病菌的毒性比率为1.4112,表现为增效作用,且盆栽防治试验防效达到71.31%,极显著高于单剂防效。可见荧光假单胞菌YG-1与啶酰菌胺复配对番茄灰霉病菌的防治具有增效作用,同时啶酰菌胺的施用量降低了50%,有效减少了杀菌剂的使用量。Adopting the indoor virulence measurement method,this paper clarified the toxic effects of 8 chemical agents and Pseudomonas fluorescent YG-1 on Botrytis cinerea;measured the biocompatibility of 8 chemical pesticides and YG-1 strain by plate count method;and screened the optimal ratio of biocontrol strains and chemical agents.The pot experiment verified further the control effect of Pseudomonas fluorescens strain YG-1 and chemical agents on tomato gray mold.The research results showed that among 8 chemical pesticides,boscalid and fludioxonil had a strong virulence effect on Botrytis cinerea,with EC50 value of 0.081 mg·L-1 and 0.075 mg·L-1,respectively.The strain YG-1 has better biocompatibility with boscalid.The optimal ratio of 1.97×108 CFU·mL-1 Pseudomonas fluorescens YG-1 strain and 0.081 mg·L-1 boscalid is 5∶5,and the toxicity ratio for Botrytis cinerea is 1.4112.The performance is synergistic,and pot control effect reaching 71.31%is extreme significantly higher than that of the single agent.The combination of Pseudomonas fluorescens YG-1 and boscalid has a synergistic control effect on Botrytis cinerea.At the same time,the application of boscalid was reduced by 50%,effectively reducing the usage amount of fungicides.

关 键 词：荧光假单胞菌 番茄灰霉病菌 联合毒力 增效作用 

分 类 号：S436.412.13[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]