The pre-mRNA splicing factor RDM16 regulates root stem cell maintenance in Arabidopsis  

作　　者：Bingsheng Lv Kongqin Hu Te Tian Kaijing Wei Feng Zhang Yuebin Jia Huiyu Tian Zhaojun Ding 

机构地区：[1]The Key Laboratory of Plant Development and Environmental Adaptation Biology,Ministry of Education,School of Life Sciences,Shandong University,Qingdao 266237,China [2]Shandong Provincial Key Laboratory of Plant Stress,College of Life Sciences,Shandong Normal University,Jinan 250014,China

出　　处：《Journal of Integrative Plant Biology》2021年第4期662-675,共14页植物学报（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China(2015CB942901 to ZD);the National Natural Science Foundation of China(Projects 31470371 and 31222005 to ZD);Project funded by China Postdoctoral Science Foundation(2018T110683 to BL);the Special Support for Post-doc Creative Funding in Shandong(201701007 to BL)。

摘　　要：Pre-mRNA(messenger RNA)splicing participates in the regulation of numerous biological processes in plants.For example,alternative splicing shapes transcriptomic responses to abiotic and biotic stress,and controls developmental programs.However,no study has revealed a role for splicing in maintaining the root stem cell niche.Here,a screen for defects in root growth in Arabidopsis thaliana identified an ethyl methane sulfonate mutant defective in pre-m RNA splicing(rdm16-4).The rdm16-4 mutant displays a short-root phenotype resulting from fewer cells in the root apical meristem.The PLETHORA1(PLT1)and PLT2 transcription factor genes are important for root development and were alternatively spliced in rdm16-4 mutants,resulting in a disordered root stem cell niche and retarded root growth.The root cap of rdm16-4 contained reduced levels of cytokinins,which promote differentiation in the developing root.This reduction was associated with the alternative splicing of genes encoding cytokinin signaling factors,such as ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN5 and ARABIDOPSIS RESPONSE REGULATORS(ARR1,ARR2,and ARR11).Furthermore,expression of the full-length coding sequence of ARR1 or exogenous cytokinin application partially rescued the short-root phenotype of rdm16-4.This reveals that the RDM16-mediated alternative splicing of cytokinin signaling components contributes to root growth.

关 键 词：CYTOKININ Pre-mRNA splicing PLETHORA RDM16 root growth stem cell 

分 类 号：Q943[生物学—植物学]