Roles of DEMETER in regulating DNA methylation in vegetative tissues and pathogen resistance  

作　　者：Wenjie Zeng Huan Huang Xueqiang Lin Chen Zhu Ken-ichi Kosami Chaofeng Huang Huiming Zhang Cheng-Guo Duan Jian-Kang Zhu Daisuke Miki 

机构地区：[1]Shanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences,the Chinese Academy of Sciences,Shanghai 210602,China [2]University of the Chinese Academy of Sciences,Shanghai 201602,China [3]Fruit Tree Research Center,Ehime Research Institute of Agriculture,Forestry and Fisheries,Ehime 7910112,Japan [4]Department of Horticulture and Landscape Architecture,Purdue University,West Lafayette,Indiana 47907,USA

出　　处：《Journal of Integrative Plant Biology》2021年第4期691-706,共16页植物学报（英文版）

基　　金：supported by a grant from the Chinese Academy of Sciences to J.K.Z.;by a grant from the National Natural Science Foundation of China(NSFC 31900482)to H.H.

摘　　要：DNA methylation is an epigenetic mark important for genome stability and gene expression.In Arabidopsis thaliana,the 5-methylcytosine DNA glycosylase/demethylase DEMETER(DME)controls active DNA demethylation during the reproductive stage;however,the lethality of loss-of-function dme mutations has made it difficult to assess DME function in vegetative tissues.Here,we edited DME using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 and created three weak dme mutants that produced a few viable seeds.We also performed central cell-specific complementation in a strong dme mutant and combined this line with mutations in the other three Arabidopsis demethylase genes to generate the dme ros1 dml2 dml3(drdd)quadruple mutant.A DNA methylome analysis showed that DME is required for DNA demethylation at hundreds of genomic regions in vegetative tissues.A transcriptome analysis of the drdd mutant revealed that DME and the other three demethylases are important for plant responses to biotic and abiotic stresses in vegetative tissues.Despite the limited role of DME in regulating DNA methylation in vegetative tissues,the dme mutants showed increased susceptibility to bacterial and fungal pathogens.Our study highlights the important functions of DME in vegetative tissues and provides valuable genetic tools for future investigations of DNA demethylation in plants.

关 键 词：active DNA demethylation Arabidopsis disease resistance DME DNA methylation 

分 类 号：Q943.2[生物学—植物学]