AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana  

作　　者：Li Ping Tang Yi Yang Hui Wang Lixin Li Le Liu Yu Liu Jinfeng Yuan Xiang Yu Zhao Klaus Palme Ying Hua Su Xugang Li 

机构地区：[1]State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tai'an 271018,China [2]Sino-German Joint Research Center on Agricultural Biology,College of Life Sciences,Shandong Agricultural University,Tai'an 271018,China [3]Institute of Biology II/Molecular Plant Physiology,Faculty of Biology,Albert-Ludwigs-University of Freiburg,Schänzlestrasse 1,Freiburg D-79104,Germany [4]Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Harbin 150040,China [5]BIOSS Centre for Biological Signalling Studies,Albert-Ludwigs-University Freiburg,Signalhaus,Schänzlestr.18,Freiburg D-79104,Germany [6]Center for Biological Systems Analysis(ZBSA),Albert-Ludwigs-University Freiburg,Freiburg D-79104,Germany

出　　处：《Journal of Integrative Plant Biology》2021年第4期737-754,共18页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31570291,31570246,31872669);Funds of Shandong“Double Tops”Program(SYL2017YSTD03);Shandong“Foreign experts double hundred”Program(WST2017008);Taishan Scholar Project;DFG(SFB 746);Excel ence Initiative of the German Federal and State Governments(EXC 294);Bundesministerium für Forschung und Technik(BMBF,MICROSYSTEMS);the Deutsches Zentrum für Luft und Raumfahrt(DLR 50WB1022);Natural Science Foundation of Heilongjiang Province(C2016002)。

摘　　要：In eukaryotes,N-ethylmaleimide-sensitive factor(NSF)is a conserved AAA+ATPase and a key component of the membrane trafficking machinery that promotes the fusion of secretory vesicles with target membranes.Here,we demonstrate that the Arabidopsis thaliana genome contains a single copy of NSF,At NSF,which plays an essential role in the regulation of leaf serration.The At NSF knock-down mutant,atnsf-1,exhibited more serrations in the leaf margin.Moreover,polar localization of the PINFORMED1(PIN1)auxin efflux transporter was diffuse around the margins of atnsf-1 leaves and root growth was inhibited in the atnsf-1 mutant.More PIN1-GFP accumulated in the intracellular compartments of atnsf-1 plants,suggesting that At NSF is required for intracellular trafficking of PIN between the endosome and plasma membrane.Furthermore,the serration phenotype was suppressed in the atnsf-1 pin1-8 double mutant,suggesting that At NSF is required for PIN1-mediated polar auxin transport to regulate leaf serration.The CUPSHAPED COTYLEDON2(CUC2)transcription factor gene is up-regulated in atnsf-1 plants and the cuc2-3 single mutant exhibits smooth leaf margins,demonstrating that At NSF also functions in the CUC2 pathway.Our results reveal that At NSF regulates the PIN1-generated auxin maxima with a CUC2-mediated feedback loop to control leaf serration.

关 键 词：At NSF AUXIN CUC2 leaf serration PIN1 vesicle trafficking 

分 类 号：Q945[生物学—植物学]